Formation of mRNA 3′ ends in eukaryotes requires the interaction of transacting factors
with cis-acting signal elements on the RNA precursor by two distinct mechanisms, one for …

Most eukaryotic mRNA precursors (premRNAs) must undergo extensive processing,
including cleavage and polyadenylation at the 3′-end. Processing at the 3′-end is …

Pre‐mRNA cleavage and polyadenylation is an essential step for 3′ end formation of
almost all protein‐coding transcripts in eukaryotes. The reaction, involving cleavage of …

Many genes have been described and characterized which result in alternative
polyadenylation site use at the 3′-end of their mRNAs based on the cellular environment …

The physiological relevance of structures within mammalian mRNAs has been elusive, as
these mRNAs are less folded in cells than in vitro and have predicted secondary structures …

The CstF polyadenylation factor is a multisubunit complex required for efficient cleavage and
polyadenylation of pre-mRNAs. Using an RNase H-mediated map** technique, we show …

Polyadenylation of mammalian mRNA precursors requires at least two signal sequences in
the RNA: the nearly invariant AAUAAA, situated 5′ to the site of polyadenylation, and a …

We previously demonstrated that a critical 35 bp region 3'of the AAUAAA is required for
rabbit (3-globln mRNA 3'end formation. Recently, we synthesized and tested sequence …

Human alpha-galactosidase A (alpha-D-galactoside galactohydrolase; EC 3.2. 1.22) is a
lysosomal hydrolase encoded by a gene localized to the chromosomal region Xq22. The …

Using a series of position and nucleotide variants of the SV40 late polyadenylation signal
we have demonstrated that three sequence elements determine the precise site of 3′-end …