Site-selective modification strategies in antibody–drug conjugates

SJ Walsh, JD Bargh, FM Dannheim… - Chemical Society …, 2021 - pubs.rsc.org
Antibody–drug conjugates (ADCs) harness the highly specific targeting capabilities of an
antibody to deliver a cytotoxic payload to specific cell types. They have garnered …

The chemistry behind ADCs

V Kostova, P Désos, JB Starck, A Kotschy - Pharmaceuticals, 2021 - mdpi.com
Combining the selective targeting of tumor cells through antigen-directed recognition and
potent cell-killing by cytotoxic payloads, antibody-drug conjugates (ADCs) have emerged in …

Diethynyl Phosphinates for Cysteine‐Selective Protein Labeling and Disulfide Rebridging

CE Stieger, L Franz, F Körlin… - Angewandte Chemie …, 2021 - Wiley Online Library
Diethynyl phosphinates were developed as bisfunctional electrophiles for the site‐selective
modification of peptides, proteins and antibodies. One of their electron‐deficient triple bonds …

Modular chemical construction of IgG-like mono-and bispecific synthetic antibodies (SynAbs)

F Thoreau, PA Szijj, MK Greene, LNC Rochet… - ACS Central …, 2023 - ACS Publications
In recent years there has been rising interest in the field of protein–protein conjugation,
especially related to bispecific antibodies (bsAbs) and their therapeutic applications. These …

All-in-one disulfide bridging enables the generation of antibody conjugates with modular cargo loading

FM Dannheim, SJ Walsh, CT Orozco, AH Hansen… - Chemical …, 2022 - pubs.rsc.org
Antibody–drug conjugates (ADCs) are valuable therapeutic entities which leverage the
specificity of antibodies to selectively deliver cytotoxins to antigen-expressing targets such …

The renaissance of chemically generated bispecific antibodies

P Szijj, V Chudasama - Nature Reviews Chemistry, 2021 - nature.com
Bispecific antibodies (bsAbs) target two different epitopes. These are an up-and-coming
class of biologics, with two such therapeutics (emicizumab and blinatumomab) FDA …

Ethynylphosphonamidates for the Rapid and Cysteine‐Selective Generation of Efficacious Antibody–Drug Conjugates

MA Kasper, A Stengl, P Ochtrop… - Angewandte Chemie …, 2019 - Wiley Online Library
Requirements for novel bioconjugation reactions for the synthesis of antibody–drug
conjugates (ADCs) are exceptionally high, since conjugation selectivity as well as the …

Leucine-rich alpha-2-glycoprotein 1 (LRG1) as a novel ADC target

F Javaid, C Pilotti, C Camilli, D Kallenberg… - RSC chemical …, 2021 - pubs.rsc.org
Leucine-rich alpha-2-glycoprotein 1 (LRG1) is present abundantly in the microenvironment
of many tumours where it contributes to vascular dysfunction, which impedes the delivery of …

Orthogonal hydroxyl functionalization of cGAMP confers metabolic stability and enables antibody conjugation

Y Lu, L You, L Li, JA Kilgore, S Liu, X Wang… - ACS Central …, 2023 - ACS Publications
cGAMP is a signaling molecule produced by the cGAS–DNA complex to establish
antimicrobial and antitumor immunity through STING. Whereas STING activation holds …

Dual reactivity disulfide bridging reagents; enabling new approaches to antibody fragment bioconjugation

A Chrzastek, IA Thanasi, JA Irving, V Chudasama… - Chemical …, 2022 - pubs.rsc.org
Disulfide bridging, also known as disulfide stapling, is a powerful strategy for the
construction of site-selective protein bioconjugates. Here we describe the first examples of a …