Single-molecule localization microscopy

M Lelek, MT Gyparaki, G Beliu, F Schueder… - Nature reviews …, 2021 - nature.com
Single-molecule localization microscopy (SMLM) describes a family of powerful imaging
techniques that dramatically improve spatial resolution over standard, diffraction-limited …

Fluorescent nanoparticles for super-resolution imaging

W Li, GS Kaminski Schierle, B Lei, Y Liu… - Chemical …, 2022 - ACS Publications
Super-resolution imaging techniques that overcome the diffraction limit of light have gained
wide popularity for visualizing cellular structures with nanometric resolution. Following the …

The cell tracking challenge: 10 years of objective benchmarking

M Maška, V Ulman, P Delgado-Rodriguez… - Nature …, 2023 - nature.com
Abstract The Cell Tracking Challenge is an ongoing benchmarking initiative that has
become a reference in cell segmentation and tracking algorithm development. Here, we …

Rationalized deep learning super-resolution microscopy for sustained live imaging of rapid subcellular processes

C Qiao, D Li, Y Liu, S Zhang, K Liu, C Liu, Y Guo… - Nature …, 2023 - nature.com
The goal when imaging bioprocesses with optical microscopy is to acquire the most
spatiotemporal information with the least invasiveness. Deep neural networks have …

Liquid–liquid phase separation drives cellular function and dysfunction in cancer

S Mehta, J Zhang - Nature Reviews Cancer, 2022 - nature.com
Cancer is a disease of uncontrollably reproducing cells. It is governed by biochemical
pathways that have escaped the regulatory bounds of normal homeostatic balance. This …

Xanthene, cyanine, oxazine and BODIPY: the four pillars of the fluorophore empire for super-resolution bioimaging

S Samanta, K Lai, F Wu, Y Liu, S Cai, X Yang… - Chemical Society …, 2023 - pubs.rsc.org
In the realm of biological research, the invention of super-resolution microscopy (SRM) has
enabled the visualization of ultrafine sub-cellular structures and their functions in live cells at …

Understanding nanoparticle endocytosis to improve targeting strategies in nanomedicine

MS de Almeida, E Susnik, B Drasler… - Chemical society …, 2021 - pubs.rsc.org
Nanoparticles (NPs) have attracted considerable attention in various fields, such as
cosmetics, the food industry, material design, and nanomedicine. In particular, the fast …

Photoswitchable diarylethenes: from molecular structures to biological applications

Z Li, X Zeng, C Gao, J Song, F He, T He, H Guo… - Coordination Chemistry …, 2023 - Elsevier
Diarylethene (DAE) photoswitches, known for their reversible photoinduced cyclization and
cycloreversion reactions, have shown great promise in various fields such as optical data …

Real-time denoising enables high-sensitivity fluorescence time-lapse imaging beyond the shot-noise limit

X Li, Y Li, Y Zhou, J Wu, Z Zhao, J Fan, F Deng… - Nature …, 2023 - nature.com
A fundamental challenge in fluorescence microscopy is the photon shot noise arising from
the inevitable stochasticity of photon detection. Noise increases measurement uncertainty …

StayGold variants for molecular fusion and membrane-targeting applications

R Ando, S Shimozono, H Ago, M Takagi, M Sugiyama… - Nature …, 2024 - nature.com
Although StayGold is a bright and highly photostable fluorescent protein, its propensity for
obligate dimer formation may hinder applications in molecular fusion and membrane …