Role of distal sites in enzyme engineering
J Gu, Y Xu, Y Nie - Biotechnology Advances, 2023 - Elsevier
The limitations associated with natural enzyme catalysis have triggered the rise of the field of
protein engineering. Traditional rational design was based on the analysis of protein …
protein engineering. Traditional rational design was based on the analysis of protein …
Hot spots-making directed evolution easier
Directed evolution has emerged as a powerful strategy to engineer various properties of
proteins. Traditional methods to construct libraries such as error-prone PCR and DNA …
proteins. Traditional methods to construct libraries such as error-prone PCR and DNA …
RING 3.0: fast generation of probabilistic residue interaction networks from structural ensembles
Residue interaction networks (RINs) are used to represent residue contacts in protein
structures. Thanks to the advances in network theory, RINs have been proved effective as an …
structures. Thanks to the advances in network theory, RINs have been proved effective as an …
Targeting SARS-CoV-2 spike protein of COVID-19 with naturally occurring phytochemicals: an in silico study for drug development
Spike glycoprotein, a class I fusion protein harboring the surface of SARS-CoV-2 (SARS-
CoV-2S), plays a seminal role in the viral infection starting from recognition of the host cell …
CoV-2S), plays a seminal role in the viral infection starting from recognition of the host cell …
Computational design of enzymes for biotechnological applications
Enzymes are the natural catalysts that execute biochemical reactions upholding life. Their
natural effectiveness has been fine-tuned as a result of millions of years of natural evolution …
natural effectiveness has been fine-tuned as a result of millions of years of natural evolution …
Coupling cell growth and biochemical pathway induction in Saccharomyces cerevisiae for production of (+)-valencene and its chemical conversion to (+)-nootkatone
Z Ye, Y Huang, B Shi, Z **ang, Z Tian, M Huang… - Metabolic …, 2022 - Elsevier
Nootkatone is a valuable, functional sesquiterpene that is widely used in food, cosmetics,
pharmaceutical, agriculture, and other fields. However, only traces of it accumulate in plants …
pharmaceutical, agriculture, and other fields. However, only traces of it accumulate in plants …
Mutability‐Landscape‐Guided Engineering of l‐Threonine Aldolase Revealing the Prelog Rule in Mediating Diastereoselectivity of C−C Bond Formation
W Zheng, Z Pu, L **ao, G Xu, L Yang… - Angewandte …, 2023 - Wiley Online Library
Abstract l‐threonine aldolase (LTA) catalyzes C− C bond synthesis with moderate
diastereoselectivity. In this study, with LTA from Cellulosilyticum sp (CpLTA) as an object, a …
diastereoselectivity. In this study, with LTA from Cellulosilyticum sp (CpLTA) as an object, a …
Computation-aided engineering of starch-debranching pullulanase from Bacillus thermoleovorans for enhanced thermostability
J Bi, S Chen, X Zhao, Y Nie, Y Xu - Applied Microbiology and …, 2020 - Springer
Pullulanases are widely used in food, medicine, and other industries because they
specifically hydrolyze α-1, 6-glycosidic linkages in starch and oligosaccharides. In addition …
specifically hydrolyze α-1, 6-glycosidic linkages in starch and oligosaccharides. In addition …
Computational Redesign of the Substrate Binding Pocket of Glutamate Dehydrogenase for Efficient Synthesis of Noncanonical l-Amino Acids
Noncanonical amino acids are attractive molecules in synthetic chemistry as important
building blocks for pharmaceuticals and agrochemicals. Asymmetric reductive amination of …
building blocks for pharmaceuticals and agrochemicals. Asymmetric reductive amination of …
Directed Evolution of l-Threonine Aldolase for the Diastereoselective Synthesis of β-Hydroxy-α-amino Acids
W Zheng, H Yu, S Fang, K Chen, Z Wang… - ACS …, 2021 - ACS Publications
l-Threonine aldolase (LTA) is an attractive tool in organic chemistry for catalyzing the
formation of β-hydroxy-α-amino acids with two chiral centers. The enzyme has a strict …
formation of β-hydroxy-α-amino acids with two chiral centers. The enzyme has a strict …