The limitations associated with natural enzyme catalysis have triggered the rise of the field of
protein engineering. Traditional rational design was based on the analysis of protein …

Directed evolution has emerged as a powerful strategy to engineer various properties of
proteins. Traditional methods to construct libraries such as error-prone PCR and DNA …

Residue interaction networks (RINs) are used to represent residue contacts in protein
structures. Thanks to the advances in network theory, RINs have been proved effective as an …

Spike glycoprotein, a class I fusion protein harboring the surface of SARS-CoV-2 (SARS-
CoV-2S), plays a seminal role in the viral infection starting from recognition of the host cell …

Enzymes are the natural catalysts that execute biochemical reactions upholding life. Their
natural effectiveness has been fine-tuned as a result of millions of years of natural evolution …

Abstract l‐threonine aldolase (LTA) catalyzes C− C bond synthesis with moderate
diastereoselectivity. In this study, with LTA from Cellulosilyticum sp (CpLTA) as an object, a …

Nootkatone is a valuable, functional sesquiterpene that is widely used in food, cosmetics,
pharmaceutical, agriculture, and other fields. However, only traces of it accumulate in plants …

Noncanonical amino acids are attractive molecules in synthetic chemistry as important
building blocks for pharmaceuticals and agrochemicals. Asymmetric reductive amination of …

Structural information can help engineer enzymes. Usually, specific amino acids in particular
regions are targeted for functional reconstruction to enhance the catalytic performance …

l-Threonine aldolase (LTA) is an attractive tool in organic chemistry for catalyzing the
formation of β-hydroxy-α-amino acids with two chiral centers. The enzyme has a strict …