Plant-inspired building blocks for future plastics

AM Vos, E Maaskant, W Post, D Bosch - Trends in Biotechnology, 2024 - cell.com
The transition from a linear fossil-based economy to a renewable circular economy requires
a new approach to produce building blocks for plastics. This provides opportunities to …

[HTML][HTML] Model-assisted CRISPRi/a library screening reveals central carbon metabolic targets for enhanced recombinant protein production in yeast

X Chen, F Li, X Li, M Otto, Y Chen, V Siewers - Metabolic Engineering, 2025 - Elsevier
Production of recombinant proteins is regarded as an important breakthrough in the field of
biomedicine and industrial biotechnology. Due to the complexity of the protein secretory …

[HTML][HTML] Knock-out of the major regulator Flo8 in Komagataella phaffii results in unique host strain performance for methanol-free recombinant protein production

C Rebnegger, M Flores-Villegas, V Kowarz, S De… - New …, 2024 - Elsevier
Flo8 is a main transcriptional regulator of flocculation and pseudohyphal growth in yeast.
Disruption of FLO8 in the popular recombinant protein production host Komagataella phaffii …

[HTML][HTML] Synthetic transcription factors establish the function of nine amino acid transactivation domains of Komagataella phaffii Mxr1

P Priya, VB Shivashankar, PN Rangarajan - Journal of Biological Chemistry, 2025 - Elsevier
The zinc finger transcription factor Mxr1 (methanol expression regulator 1) of the
methylotrophic yeast Komagataella phaffii (formerly Pichia pastoris) harbors a DNA-binding …

Precision Fermentation as an Alternative to Animal Protein, a Review

MM Knychala, LA Boing, JL Ienczak, D Trichez… - Fermentation, 2024 - mdpi.com
The global food production system faces several challenges, including significant
environmental impacts due to traditional agricultural practices. The rising demands of …

Characterization of the exopolysaccharides produced by the industrial yeast Komagataella phaffii

A Fischer, V Castagna, S Omer… - Journal of Industrial …, 2024 - academic.oup.com
The yeast Komagataella phaffii has become a popular host strain among biotechnology start-
up companies for producing recombinant proteins for food and adult nutrition applications …

Yeast-based production platform for potent and stable heavy chain-only antibodies

C Lonigro, H Eeckhaut, RA Symakani, K Roose… - bioRxiv, 2024 - biorxiv.org
Monoclonal antibodies are the leading drug of the biopharmaceutical market because of
their high specificity and tolerability, but the current CHO-based manufacturing platform …

Molecular, Genetic, and Process Approaches for Improving Secreted Pharmaceutical Protein Quality in Komagataella phaffii

Y Yang - 2024 - dspace.mit.edu
Biopharmaceutical products constitute a significant portion of the global bioeconomy.
Compared to traditional synthetic small-molecule drugs, recombinant therapeutic proteins …