This review analyzes a development in biochemistry, enzymology and biotechnology that
originally came as a surprise. Following the establishment of directed evolution of …

Directed evolution has emerged as a powerful strategy to engineer various properties of
proteins. Traditional methods to construct libraries such as error-prone PCR and DNA …

In the area of human-made innovations to improve the quality of life, biocatalysis has
already had a great impact and contributed enormously to a growing number of catalytic …

The bacterial metabolic enzyme 1-deoxy-d-xylulose-5-phosphate synthase (DXPS)
catalyzes the thiamin diphosphate (ThDP)-dependent formation of DXP from pyruvate and d …

In enzyme engineering, the main targets for enhancing properties are enzyme activity,
stereoselective specificity, stability, substrate range, and the development of unique …

Advances in directed evolution have led to an exploration of new and important chemical
transformations; however, many of these efforts still rely on the use of low-throughput …

Improving the range of substrates accepted by enzymes with high catalytic activity remains
an important goal for the industrialisation of biocatalysis. Many enzymes catalyse two …

The bacterial enzyme 1-deoxy-d-xylulose 5-phosphate synthase (DXPS) catalyzes the
formation of DXP from pyruvate and d-glyceraldehyde 3-phosphate (d-GAP) in a thiamin …

A number of enzymes important for biocatalyst development or as drug targets are
associated with a pH shift during their catalytic reaction, owing to the concommitant release …

The thermostable transketolase from Geobacillus stearothermophilus (TKgst) was
successfully engineered for the synthesis of aliphatic acyloins with varying carbon backbone …