Prime editing for precise and highly versatile genome manipulation
Programmable gene-editing tools have transformed the life sciences and have shown
potential for the treatment of genetic disease. Among the CRISPR–Cas technologies that …
potential for the treatment of genetic disease. Among the CRISPR–Cas technologies that …
Assessing and advancing the safety of CRISPR-Cas tools: from DNA to RNA editing
CRISPR-Cas gene editing has revolutionized experimental molecular biology over the past
decade and holds great promise for the treatment of human genetic diseases. Here we …
decade and holds great promise for the treatment of human genetic diseases. Here we …
Precise integration of large DNA sequences in plant genomes using PrimeRoot editors
A technique for chromosomal insertion of large DNA segments is much needed in plant
breeding and synthetic biology to facilitate the introduction of desired agronomic traits and …
breeding and synthetic biology to facilitate the introduction of desired agronomic traits and …
Prime editing: advances and therapeutic applications
Clustered regularly interspaced short palindromic repeats-associated protein 9 (CRISPR–
Cas)-mediated genome editing has revolutionized biomedical research and will likely …
Cas)-mediated genome editing has revolutionized biomedical research and will likely …
NPR1, a key immune regulator for plant survival under biotic and abiotic stresses
Nonexpressor of pathogenesis-related genes 1 (NPR1) was discovered in Arabidopsis as
an activator of salicylic acid (SA)-mediated immune responses nearly 30 years ago. How …
an activator of salicylic acid (SA)-mediated immune responses nearly 30 years ago. How …
[HTML][HTML] Phage-assisted evolution and protein engineering yield compact, efficient prime editors
Prime editing enables a wide variety of precise genome edits in living cells. Here we use
protein evolution and engineering to generate prime editors with reduced size and improved …
protein evolution and engineering to generate prime editors with reduced size and improved …
Tuning plant phenotypes by precise, graded downregulation of gene expression
The ability to control gene expression and generate quantitative phenotypic changes is
essential for breeding new and desired traits into crops. Here we report an efficient, facile …
essential for breeding new and desired traits into crops. Here we report an efficient, facile …
CRISPR technologies for genome, epigenome and transcriptome editing
Our ability to edit genomes lags behind our capacity to sequence them, but the growing
understanding of CRISPR biology and its application to genome, epigenome and …
understanding of CRISPR biology and its application to genome, epigenome and …
Transposase-assisted target-site integration for efficient plant genome engineering
The current technologies to place new DNA into specific locations in plant genomes are low
frequency and error-prone, and this inefficiency hampers genome-editing approaches to …
frequency and error-prone, and this inefficiency hampers genome-editing approaches to …
[HTML][HTML] CRISPR/Cas-mediated plant genome editing: outstanding challenges a decade after implementation
The discovery of the CRISPR/Cas genome-editing system has revolutionized our
understanding of the plant genome. CRISPR/Cas has been used for over a decade to …
understanding of the plant genome. CRISPR/Cas has been used for over a decade to …