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Ultrafast magic angle spinning solid-state NMR spectroscopy: Advances in methodology and applications
Y Nishiyama, G Hou, V Agarwal, Y Su… - Chemical …, 2022 - ACS Publications
Solid-state NMR spectroscopy is one of the most commonly used techniques to study the
atomic-resolution structure and dynamics of various chemical, biological, material, and …
atomic-resolution structure and dynamics of various chemical, biological, material, and …
Solid-state NMR: Methods for biological solids
S Ahlawat, KR Mote, NA Lakomek… - Chemical Reviews, 2022 - ACS Publications
In the last two decades, solid-state nuclear magnetic resonance (ssNMR) spectroscopy has
transformed from a spectroscopic technique investigating small molecules and industrial …
transformed from a spectroscopic technique investigating small molecules and industrial …
[HTML][HTML] The structural basis of secondary active transport mechanisms
LR Forrest, R Krämer, C Ziegler - Biochimica et Biophysica Acta (BBA) …, 2011 - Elsevier
Secondary active transporters couple the free energy of the electrochemical potential of one
solute to the transmembrane movement of another. As a basic mechanistic explanation for …
solute to the transmembrane movement of another. As a basic mechanistic explanation for …
Antiparallel EmrE exports drugs by exchanging between asymmetric structures
EA Morrison, GT DeKoster, S Dutta, R Vafabakhsh… - Nature, 2012 - nature.com
Small multidrug resistance transporters provide an ideal system to study the minimal
requirements for active transport. EmrE is one such transporter in Escherichia coli. It exports …
requirements for active transport. EmrE is one such transporter in Escherichia coli. It exports …
Five decades of homonuclear dipolar decoupling in solid-state NMR: Status and outlook
It has been slightly more than fifty years since the first homonuclear spin decoupling
scheme, Lee-Goldburg decoupling, was proposed for removing homonuclear dipolar …
scheme, Lee-Goldburg decoupling, was proposed for removing homonuclear dipolar …
Proton-detected scalar coupling based assignment strategies in MAS solid-state NMR spectroscopy applied to perdeuterated proteins
Assignment of proteins in MAS (magic angle spinning) solid-state NMR relies so far on
correlations among heteronuclei. This strategy is based on well dispersed resonances in the …
correlations among heteronuclei. This strategy is based on well dispersed resonances in the …
Intrinsic conformational plasticity of native EmrE provides a pathway for multidrug resistance
MK Cho, A Gayen, JR Banigan… - Journal of the …, 2014 - ACS Publications
EmrE is a multidrug resistance efflux pump with specificity to a wide range of antibiotics and
antiseptics. To obtain atomic-scale insight into the attributes of the native state that encodes …
antiseptics. To obtain atomic-scale insight into the attributes of the native state that encodes …
Detecting substrates bound to the secondary multidrug efflux pump EmrE by DNP-enhanced solid-state NMR
YS Ong, A Lakatos, J Becker-Baldus… - Journal of the …, 2013 - ACS Publications
Escherichia coli EmrE, a homodimeric multidrug antiporter, has been suggested to offer a
convenient paradigm for secondary transporters due to its small size. It contains four …
convenient paradigm for secondary transporters due to its small size. It contains four …
[HTML][HTML] Effects of nucleotide binding to LmrA: A combined MAS-NMR and solution NMR study
UA Hellmich, L Mönkemeyer, S Velamakanni… - … et Biophysica Acta (BBA …, 2015 - Elsevier
ABC transporters are fascinating examples of fine-tuned molecular machines that use the
energy from ATP hydrolysis to translocate a multitude of substrates across biological …
energy from ATP hydrolysis to translocate a multitude of substrates across biological …
Efficient and stable reconstitution of the ABC transporter BmrA for solid-state NMR studies
B Kunert, C Gardiennet, D Lacabanne… - Frontiers in molecular …, 2014 - frontiersin.org
We present solid-state NMR sample preparation and first 2D spectra of the Bacillus subtilis
ATP-binding cassette (ABC) transporter BmrA, a membrane protein involved in multidrug …
ATP-binding cassette (ABC) transporter BmrA, a membrane protein involved in multidrug …