[HTML][HTML] Next generation and other sequencing technologies in diagnostic microbiology and infectious diseases

EE Hilt, P Ferrieri - Genes, 2022‏ - mdpi.com
Next-generation sequencing (NGS) technologies have become increasingly available for
use in the clinical microbiology diagnostic environment. There are three main applications of …

The epidemiology, pathogenesis, transmission, diagnosis, and management of multidrug-resistant, extensively drug-resistant, and incurable tuberculosis

K Dheda, T Gumbo, G Maartens, KE Dooley… - The lancet Respiratory …, 2017‏ - thelancet.com
Global tuberculosis incidence has declined marginally over the past decade, and
tuberculosis remains out of control in several parts of the world including Africa and Asia …

Precise phylogenetic analysis of microbial isolates and genomes from metagenomes using PhyloPhlAn 3.0

F Asnicar, AM Thomas, F Beghini, C Mengoni… - Nature …, 2020‏ - nature.com
Microbial genomes are available at an ever-increasing pace, as cultivation and sequencing
become cheaper and obtaining metagenome-assembled genomes (MAGs) becomes more …

CRISPRi chemical genetics and comparative genomics identify genes mediating drug potency in Mycobacterium tuberculosis

S Li, NC Poulton, JS Chang, ZA Azadian… - Nature …, 2022‏ - nature.com
Mycobacterium tuberculosis (Mtb) infection is notoriously difficult to treat. Treatment efficacy
is limited by Mtb's intrinsic drug resistance, as well as its ability to evolve acquired resistance …

The 2021 WHO catalogue of Mycobacterium tuberculosis complex mutations associated with drug resistance: a genotypic analysis

TM Walker, P Miotto, CU Köser, PW Fowler… - The Lancet …, 2022‏ - thelancet.com
Background Molecular diagnostics are considered the most promising route to achievement
of rapid, universal drug susceptibility testing for Mycobacterium tuberculosis complex …

Building a tuberculosis-free world: The Lancet Commission on tuberculosis

MJA Reid, N Arinaminpathy, A Bloom, BR Bloom… - The Lancet, 2019‏ - thelancet.com
Executive summary Tuberculosis can be treated, prevented, and cured. Rapid, sustained
declines in tuberculosis deaths in many countries during the past 50 years provide …

Genome-based prediction of bacterial antibiotic resistance

M Su, SW Satola, TD Read - Journal of clinical microbiology, 2019‏ - journals.asm.org
Clinical microbiology has long relied on growing bacteria in culture to determine
antimicrobial susceptibility profiles, but the use of whole-genome sequencing for antibiotic …

Clinical use of whole genome sequencing for Mycobacterium tuberculosis

AA Witney, CA Cosgrove, A Arnold, J Hinds, NG Stoker… - BMC medicine, 2016‏ - Springer
Drug-resistant tuberculosis (TB) remains a major challenge to global health and to
healthcare in the UK. In 2014, a total of 6,520 cases of TB were recorded in England, of …

[HTML][HTML] Genomics for emerging pathogen identification and monitoring: Prospects and obstacles

V Vashisht, A Vashisht, AK Mondal, J Farmaha… - …, 2023‏ - mdpi.com
Emerging infectious diseases (EIDs) pose an increasingly significant global burden, driven
by urbanization, population explosion, global travel, changes in human behavior, and …

Stepwise pathogenic evolution of Mycobacterium abscessus

JM Bryant, KP Brown, S Burbaud, I Everall… - Science, 2021‏ - science.org
INTRODUCTION Nearly all mycobacterial species are free-living environmental
saprophytes. A few, such as Mycobacterium tuberculosis, have evolved to cause …