Synthetic biology: bottom-up assembly of molecular systems
The bottom-up assembly of biological and chemical components opens exciting
opportunities to engineer artificial vesicular systems for applications with previously unmet …
opportunities to engineer artificial vesicular systems for applications with previously unmet …
Cell-free protein synthesis: a promising option for future drug development
SK Dondapati, M Stech, A Zemella, S Kubick - BioDrugs, 2020 - Springer
Proteins are the main source of drug targets and some of them possess therapeutic potential
themselves. Among them, membrane proteins constitute approximately 50% of the major …
themselves. Among them, membrane proteins constitute approximately 50% of the major …
[HTML][HTML] Membrane protein synthesis in cell-free systems: From bio-mimetic systems to bio-membranes
R Sachse, SK Dondapati, SF Fenz, T Schmidt, S Kubick - FEBS letters, 2014 - Elsevier
When taking up the gauntlet of studying membrane protein functionality, scientists are
provided with a plethora of advantages, which can be exploited for the synthesis of these …
provided with a plethora of advantages, which can be exploited for the synthesis of these …
Diblock copolymers enhance folding of a mechanosensitive membrane protein during cell-free expression
The expression and integration of membrane proteins into vesicle membranes is a critical
step in the design of cell-mimetic biosensors, bioreactors, and artificial cells. While …
step in the design of cell-mimetic biosensors, bioreactors, and artificial cells. While …
Mitochondrial K+ channels and their implications for disease mechanisms
The field of mitochondrial ion channels underwent a rapid development during the last
decade, thanks to the molecular identification of some of the nuclear-encoded organelle …
decade, thanks to the molecular identification of some of the nuclear-encoded organelle …
Cell-free synthesis of connexin 43-integrated exosome-mimetic nanoparticles for siRNA delivery
M Lu, X Zhao, H **ng, H Liu, L Lang, T Yang, Z Xun… - Acta biomaterialia, 2019 - Elsevier
Exosomes are naturally secreted nanovesicles that have emerged as a promising
therapeutic nanodelivery platform, due to their specific composition and biological …
therapeutic nanodelivery platform, due to their specific composition and biological …
[HTML][HTML] Membrane protein production in Escherichia coli cell-free lysates
E Henrich, C Hein, V Dötsch, F Bernhard - FEBS letters, 2015 - Elsevier
Cell-free protein production has become a core technology in the rapidly spreading field of
synthetic biology. In particular the synthesis of membrane proteins, highly problematic …
synthetic biology. In particular the synthesis of membrane proteins, highly problematic …
The E. coli S30 lysate proteome: A prototype for cell-free protein production
D Foshag, E Henrich, E Hiller, M Schäfer, C Kerger… - New …, 2018 - Elsevier
Protein production using processed cell lysates is a core technology in synthetic biology and
these systems are excellent to produce difficult toxins or membrane proteins. However, the …
these systems are excellent to produce difficult toxins or membrane proteins. However, the …
Promiscuous targeting of polytopic membrane proteins to SecYEG or YidC by the Escherichia coli signal recognition particle
Protein insertion into the bacterial inner membrane is facilitated by SecYEG or YidC.
Although SecYEG most likely constitutes the major integration site, small membrane proteins …
Although SecYEG most likely constitutes the major integration site, small membrane proteins …
[HTML][HTML] Biogenesis of inner membrane proteins in Escherichia coli
The inner membrane proteome of the model organism Escherichia coli is composed of inner
membrane proteins, lipoproteins and peripherally attached soluble proteins. Our knowledge …
membrane proteins, lipoproteins and peripherally attached soluble proteins. Our knowledge …