Thiomethyltetrazines Are Reversible Covalent Cysteine Warheads Whose Dynamic Behavior can be “Switched Off” via Bioorthogonal Chemistry Inside Live Cells
Electrophilic small molecules that can reversibly modify proteins are of growing interest in
drug discovery. However, the ability to study reversible covalent probes in live cells can be …
drug discovery. However, the ability to study reversible covalent probes in live cells can be …
One-pot method for preparing DNA, RNA, and protein for multiomics analysis
Typical multiomics studies employ separate methods for DNA, RNA, and protein sample
preparation, which is labor intensive, costly, and prone to sampling bias. We describe a …
preparation, which is labor intensive, costly, and prone to sampling bias. We describe a …
IP-to-MS: An Unbiased Workflow for Antigen Profiling
Immunoprecipitation is among the most widely utilized methods in biomedical research, with
applications that include the identification of antibody targets and associated proteins. The …
applications that include the identification of antibody targets and associated proteins. The …
Influence of Aldehyde Dehydrogenase Inhibition on Stemness of Endometrial Cancer Stem Cells
B Serambeque, C Mestre, G Correia-Barros, R Teixo… - Cancers, 2024 - mdpi.com
Simple Summary Cancer stem cells (CSCs) are responsible for tumour initiation, growth,
dissemination, and resistance to therapy. In this study, we intended to evaluate the effect of …
dissemination, and resistance to therapy. In this study, we intended to evaluate the effect of …
Expanding Glycomic Investigations through Thiol-Derivatized Glycans
RD Hurst, A Nieves, M Brichacek - Molecules, 2023 - mdpi.com
N-(2-thioethyl)-2-aminobenzamide (TEAB), a novel glycan auxiliary, was synthesized and its
utility was evaluated. The auxiliary was conjugated to glycans by reductive amination with …
utility was evaluated. The auxiliary was conjugated to glycans by reductive amination with …
Affinity Bioorthogonal Chemistry (ABC) Tags for Site‐Selective Conjugation, On‐Resin Protein‐Protein Coupling, and Purification of Protein Conjugates
The site‐selective functionalization of proteins has broad application in chemical biology,
but can be limited when mixtures result from incomplete conversion or the formation of …
but can be limited when mixtures result from incomplete conversion or the formation of …
Affinity Bioorthogonal Chemistry (ABC) Tags for Site-selective Conjugation, On-resin Protein-Protein Coupling, and Purification of Protein Conjugates
The site-selective functionalization of proteins has broad application in chemical biology, but
can be limited when mixtures result from incomplete conversion or the formation of protein …
can be limited when mixtures result from incomplete conversion or the formation of protein …
[PDF][PDF] MT-Trypsin mediated rapid in-gel digestion with trypsin removal
S Yablonska, S Biedka, JS Minden - impactproteomics.com
Utilizing click chemistry technology, we developed an in-gel digestion protocol that enables
rapid, twohour enzymatic digestion of proteins. This workflow includes aspects of a …
rapid, twohour enzymatic digestion of proteins. This workflow includes aspects of a …