Inhibitors of bacterial immune systems: discovery, mechanisms and applications

D Mayo-Muñoz, R Pinilla-Redondo… - Nature Reviews …, 2024‏ - nature.com
To contend with the diversity and ubiquity of bacteriophages and other mobile genetic
elements, bacteria have developed an arsenal of immune defence mechanisms. Bacterial …

Controlling and enhancing CRISPR systems

H Shivram, BF Cress, GJ Knott, JA Doudna - Nature chemical biology, 2021‏ - nature.com
Many bacterial and archaeal organisms use clustered regularly interspaced short
palindromic repeats–CRISPR associated (CRISPR–Cas) systems to defend themselves …

Structure-based functional mechanisms and biotechnology applications of anti-CRISPR proteins

N Jia, DJ Patel - Nature Reviews Molecular Cell Biology, 2021‏ - nature.com
CRISPR loci and Cas proteins provide adaptive immunity in prokaryotes against invading
bacteriophages and plasmids. In response, bacteriophages have evolved a broad spectrum …

Discovery of multiple anti-CRISPRs highlights anti-defense gene clustering in mobile genetic elements

R Pinilla-Redondo, S Shehreen, ND Marino… - Nature …, 2020‏ - nature.com
Many prokaryotes employ CRISPR–Cas systems to combat invading mobile genetic
elements (MGEs). In response, some MGEs have developed strategies to bypass immunity …

Phage anti-CRISPR control by an RNA-and DNA-binding helix–turn–helix protein

N Birkholz, K Kamata, M Feussner, ME Wilkinson… - Nature, 2024‏ - nature.com
In all organisms, regulation of gene expression must be adjusted to meet cellular
requirements and frequently involves helix–turn–helix (HTH) domain proteins. For instance …

Structures and strategies of anti-CRISPR-mediated immune suppression

T Wiegand, S Karambelkar… - Annual review of …, 2020‏ - annualreviews.org
More than 50 protein families have been identified that inhibit CRISPR (clustered regularly
interspaced short palindromic repeats)-Cas-mediated adaptive immune systems. Here, we …

Unveil the secret of the bacteria and phage arms race

Y Wang, H Fan, Y Tong - International Journal of Molecular Sciences, 2023‏ - mdpi.com
Bacteria have developed different mechanisms to defend against phages, such as
preventing phages from being adsorbed on the surface of host bacteria; through the …

Anti-CRISPRs go viral: the infection biology of CRISPR-Cas inhibitors

Y Li, J Bondy-Denomy - Cell host & microbe, 2021‏ - cell.com
Bacteriophages encode diverse anti-CRISPR (Acr) proteins that inhibit CRISPR-Cas
immunity during infection of their bacterial hosts. Although detailed mechanisms have been …

Antibiotics that affect translation can antagonize phage infectivity by interfering with the deployment of counter-defenses

BJ Pons, T Dimitriu, ER Westra, S van Houte - Proceedings of the National …, 2023‏ - pnas.org
It is becoming increasingly clear that antibiotics can both positively and negatively impact
the infectivity of bacteriophages (phage), but the underlying mechanisms often remain …

Discovery of potent and versatile CRISPR–Cas9 inhibitors engineered for chemically controllable genome editing

G Song, F Zhang, C Tian, X Gao, X Zhu… - Nucleic acids …, 2022‏ - academic.oup.com
Anti-CRISPR (Acr) proteins are encoded by many mobile genetic elements (MGEs) such as
phages and plasmids to combat CRISPR–Cas adaptive immune systems employed by …