Assessing the effects of primer specificity on eliminating numt coamplification in DNA barcoding: a case study from Orthoptera (Arthropoda: Insecta)

MJ Moulton, H Song, MF Whiting - Molecular Ecology …, 2010 - Wiley Online Library
DNA barcoding is a diagnostic method of species identification based on sequencing a short
mitochondrial DNA fragment of cytochrome oxidase I (COI), but its ability to correctly …

Identification of Viable Helicobacter pylori in Drinking Water Supplies by Cultural and Molecular Techniques

P Santiago, Y Moreno, MA Ferrús - Helicobacter, 2015 - Wiley Online Library
Background Helicobacter pylori is one of the most common causes of chronic bacterial
infection in humans, directly related to peptic ulcer and gastric cancer. It has been suggested …

Transcriptional and enzymatic profiling of Pleurotus ostreatus laccase genes in submerged and solid-state fermentation cultures

R Castanera, G Pérez, A Omarini, M Alfaro… - Applied and …, 2012 - journals.asm.org
The genome of the white rot basidiomycete Pleurotus ostreatus includes 12 phenol oxidase
(laccase) genes. In this study, we examined their expression profiles in different fungal …

Qualitative and quantitative methodologies for determination of airborne microorganisms at concentrated animal-feeding operations

RS Dungan, AB Leytem - World Journal of Microbiology and …, 2009 - Springer
The generation of airborne microorganisms from concentrated animal-feeding operations
(CAFOs) is a concern from a human and animal health perspective. To better understand the …

Small-scale total DNA extraction from bacteria and yeast for PCR applications

JL Ruiz-Barba, A Maldonado-Barragán… - 2005 - digital.csic.es
In this study, we describe a very simple and rapid method for extraction of both plasmid and
chromosomal DNA from colonies or pellets of bacteria and yeast that is suitable for …

A rapid and low-cost PCR thermal cycler for low resource settings

G Wong, I Wong, K Chan, Y Hsieh, S Wong - PLoS one, 2015 - journals.plos.org
Background Many modern molecular diagnostic assays targeting nucleic acids are typically
confined to developed countries or to the national reference laboratories of develo** …

A rapid and low-cost PCR thermal cycler for infectious disease diagnostics

K Chan, PY Wong, P Yu, J Hardick, KY Wong… - PLoS …, 2016 - journals.plos.org
The ability to make rapid diagnosis of infectious diseases broadly available in a portable,
low-cost format would mark a great step forward in global health. Many molecular diagnostic …

[HTML][HTML] Detection of the sickle hemoglobin allele using a surface plasmon resonance based biosensor

G Breveglieri, E D'Aversa, LC Cosenza… - Sensors and Actuators B …, 2019 - Elsevier
Abstract Sickle Cell Disease (SCD) is a monogenic hereditary blood disorder caused by a
single point mutation (β S) in the β globin gene resulting in an abnormal hemoglobin (HbS) …

Molecular characterization of selected dermatophytes and their identification by electrophoretic mutation scanning

C Cafarchia, D Otranto, S Weigl, BE Campbell… - …, 2009 - Wiley Online Library
Dermatophytes are fungi that can be contagious and cause infections in the keratinized skin
of mammals, including humans. The etiological diagnosis of dermatophytosis relies on a …