Epigenetics, fragmentomics, and topology of cell-free DNA in liquid biopsies

YMD Lo, DSC Han, P Jiang, RWK Chiu - Science, 2021 - science.org
BACKGROUND Liquid biopsies that are based on analysis of cell-free DNA from plasma
offer diagnostic information that is otherwise accessible conventionally through invasive …

DNA sequencing at 40: past, present and future

J Shendure, S Balasubramanian, GM Church… - Nature, 2017 - nature.com
This review commemorates the 40th anniversary of DNA sequencing, a period in which we
have already witnessed multiple technological revolutions and a growth in scale from a few …

An ultrasensitive method for quantitating circulating tumor DNA with broad patient coverage

AM Newman, SV Bratman, J To, JF Wynne… - Nature medicine, 2014 - nature.com
Circulating tumor DNA (ctDNA) is a promising biomarker for noninvasive assessment of
cancer burden, but existing ctDNA detection methods have insufficient sensitivity or patient …

Liquid biopsies: genoty** circulating tumor DNA

LA Diaz Jr, A Bardelli - Journal of clinical oncology, 2014 - ascopubs.org
Genoty** tumor tissue in search of somatic genetic alterations for actionable information
has become routine practice in clinical oncology. Although these sequence alterations are …

Cell-free DNA comprises an in vivo nucleosome footprint that informs its tissues-of-origin

MW Snyder, M Kircher, AJ Hill, RM Daza, J Shendure - Cell, 2016 - cell.com
Nucleosome positioning varies between cell types. By deep sequencing cell-free DNA
(cfDNA), isolated from circulating blood plasma, we generated maps of genome-wide in vivo …

The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments

SA Bustin, V Benes, JA Garson, J Hellemans, J Huggett… - 2009 - academic.oup.com
Background: Currently, a lack of consensus exists on how best to perform and interpret
quantitative real-time PCR (qPCR) experiments. The problem is exacerbated by a lack of …

High-throughput droplet digital PCR system for absolute quantitation of DNA copy number

BJ Hindson, KD Ness, DA Masquelier… - Analytical …, 2011 - ACS Publications
Digital PCR enables the absolute quantitation of nucleic acids in a sample. The lack of
scalable and practical technologies for digital PCR implementation has hampered the …

Analytical and clinical validation of a microbial cell-free DNA sequencing test for infectious disease

TA Blauwkamp, S Thair, MJ Rosen, L Blair… - Nature …, 2019 - nature.com
Thousands of pathogens are known to infect humans, but only a fraction are readily
identifiable using current diagnostic methods. Microbial cell-free DNA sequencing offers the …

The molecular evolution of acquired resistance to targeted EGFR blockade in colorectal cancers

LA Diaz Jr, RT Williams, J Wu, I Kinde, JR Hecht… - Nature, 2012 - nature.com
Colorectal tumours that are wild type for KRAS are often sensitive to EGFR blockade, but
almost always develop resistance within several months of initiating therapy,. The …

[HTML][HTML] Noninvasive prenatal screening for fetal aneuploidy, 2016 update: a position statement of the American College of Medical Genetics and Genomics

AR Gregg, BG Skotko, JL Benkendorf, KG Monaghan… - Genetics in …, 2016 - Elsevier
Disclaimer: This statement is designed primarily as an educational resource for clinicians to
help them provide quality medical services. Adherence to this statement is completely …