Single-photon avalanche diode (SPAD) arrays are solid-state detectors that offer imaging
capabilities at the level of individual photons, with unparalleled photon counting and time …

BACKGROUND Biomolecular mechanisms are typically inferred from static structural
“snapshots” obtained by x-ray crystallography, nuclear magnetic resonance (NMR) …

Fluorescence resonance energy transfer (FRET) is a powerful spectroscopic method for
measuring distances in the 2–8 nm range. Often, conformational changes and molecular …

Single-molecule FRET (smFRET) has become a mainstream technique for studying
biomolecular structural dynamics. The rapid and wide adoption of smFRET experiments by …

Substrate-binding proteins (SBPs) are associated with ATP-binding cassette importers and
switch from an open to a closed conformation upon substrate binding, providing specificity …

Summary α-Synuclein plays an important role in synaptic functions by interacting with
synaptic vesicle membrane, while its oligomers and fibrils are associated with several …

Over the past decades, single-molecule and super-resolution microscopy have advanced
and represent essential tools for life science research. There is, however, a growing gap …

Single molecule Förster resonance energy transfer (smFRET) is a unique biophysical
approach for studying conformational dynamics in biomacromolecules. Photon-by-photon …

Single-photon detection is an invaluable tool for many applications ranging from basic
research to consumer electronics. In this respect, the Single Photon Avalanche Diode …

Conformational changes of proteins and other biomolecules play a fundamental role in their
functional mechanism. Single pair Förster resonance energy transfer (spFRET) offers the …