In the 30 years since its discovery, tyrosine phosphorylation has emerged as a
fundamentally important mechanism of signal transduction and regulation in all eukaryotic …

Emerin, a conserved LEM-domain protein, is among the few nuclear membrane proteins for
which extensive basic knowledge—biochemistry, partners, functions, localizations …

Cell signaling mechanisms often transmit information via posttranslational protein
modifications, most importantly reversible protein phosphorylation. Here we develop and …

Protein phosphorylation plays a key role in vasopressin signaling in the renal-collecting
duct. Large-scale identification and quantification of phosphorylation events triggered by …

Proteomics is the large-scale study of proteins, particularly their expression, structures and
functions. This still-emerging combination of technologies aims to describe and characterize …

Reversible protein phosphorylation serves as a basis for regulating a number of cellular
processes. Aberrant activation of kinase signaling pathways is commonly associated with …

Chemical derivatisation of functional groups has proved popular since the beginning of
organic mass spectrometry as a means to enhance the stability and volatility of the analytes …

Several mass spectrometry-based assays have emerged for the quantitative profiling of
cellular tyrosine phosphorylation. Ideally, these methods should reveal the exact sites of …

Using recombinant bead-conjugated emerin, we affinity-purified seven proteins from HeLa
cell nuclear lysates that bind emerin either directly or indirectly. These proteins were …

Epidermal growth factor receptors (ErbB1–4) are oncogenic receptor tyrosine kinases
(RTKs) that regulate diverse cellular processes. In this study, we combine measurement and …