Production of HMOs using microbial hosts—from cell engineering to large scale production

K Bych, MH Mikš, T Johanson, MJ Hederos… - Current opinion in …, 2019 - Elsevier
Highlights•Since 2015, human milk oligosaccharides (HMOs) have become commercially
available.•Major infant formula manufacturers have introduced HMOs in their products.• …

Recent advances on 2′-fucosyllactose: physiological properties, applications, and production approaches

Y Zhu, L Wan, W Li, D Ni, W Zhang… - Critical Reviews in …, 2022 - Taylor & Francis
Abstract The trisaccharide, 2′-fucosyllactose (Fucα1-2Galβ1-4Glc; 2′-FL), is the most
abundant oligosaccharide in human milk. It has numerous significant biological properties …

Strategies for enhancing microbial production of 2′-fucosyllactose, the most abundant human milk oligosaccharide

Y Liu, Y Zhu, H Wang, L Wan, W Zhang… - Journal of Agricultural …, 2022 - ACS Publications
Human milk oligosaccharides (HMOs), a group of structurally diverse unconjugated glycans
in breast milk, act as important prebiotics and have plenty of unique health effects for …

Combinatorial metabolic engineering and tolerance evolving of Escherichia coli for high production of 2′-fucosyllactose

X Sun, Z Peng, C Li, Y Zheng, J Zong, F Lu, Y Li… - Bioresource …, 2023 - Elsevier
Fucosyllactose (2′-FL) is an important functional ingredient of advanced infant formula.
Here, Escherichia coli MG1655 was engineered for achieving high 2′-FL production. The …

Construction of Escherichia coli strains with chromosomally integrated expression cassettes for the synthesis of 2-fucosyllactose

F Baumgärtner, L Seitz, GA Sprenger… - Microbial Cell …, 2013 - Springer
Abstract Background The trisaccharide 2′-fucosyllactose (2′-FL) is one of the most
abundant oligosaccharides found in human milk. Due to its prebiotic and anti-infective …

Biotechnological production of fucosylated human milk oligosaccharides: Prokaryotic fucosyltransferases and their use in biocatalytic cascades or whole cell …

B Petschacher, B Nidetzky - Journal of Biotechnology, 2016 - Elsevier
Human milk oligosaccharides (HMOs) constitute a class of complex carbohydrates unique to
mother's milk and are strongly correlated to the health benefits of breastfeeding in infants …

Whole cell biosynthesis of a functional oligosaccharide, 2′-fucosyllactose, using engineered Escherichia coli

WH Lee, P Pathanibul, J Quarterman, JH Jo… - Microbial Cell …, 2012 - Springer
Abstract Background 2'-Fucosyllactose (2-FL) is a functional oligosaccharide present in
human milk which protects against the infection of enteric pathogens. Because 2-FL can be …

Enhanced production of 2′-fucosyllactose in engineered Escherichia coli BL21star (DE3) by modulation of lactose metabolism and fucosyltransferase

YW Chin, JY Kim, WH Lee, JH Seo - Journal of Biotechnology, 2015 - Elsevier
Fucosyllactose (2-FL) is one of most abundant functional oligosaccharides in human milk,
which is involved in many biological functions for human health. To date, most microbial …

Biotechnological production of human milk oligosaccharides

NS Han, TJ Kim, YC Park, J Kim, JH Seo - Biotechnology advances, 2012 - Elsevier
Human milk contains a large variety of oligosaccharides (HMOs) that have the potential to
modulate the gut flora, affect different gastrointestinal functions, and influence inflammatory …

Engineered microbial routes for human milk oligosaccharides synthesis

M Lu, I Mosleh, A Abbaspourrad - ACS Synthetic Biology, 2021 - ACS Publications
Human milk oligosaccharides (HMOs) are one of the important ingredients in human milk,
which have attracted great interest due to their beneficial effect on the health of newborns …