Production of HMOs using microbial hosts—from cell engineering to large scale production
K Bych, MH Mikš, T Johanson, MJ Hederos… - Current opinion in …, 2019 - Elsevier
Highlights•Since 2015, human milk oligosaccharides (HMOs) have become commercially
available.•Major infant formula manufacturers have introduced HMOs in their products.• …
available.•Major infant formula manufacturers have introduced HMOs in their products.• …
Recent advances on 2′-fucosyllactose: physiological properties, applications, and production approaches
Y Zhu, L Wan, W Li, D Ni, W Zhang… - Critical Reviews in …, 2022 - Taylor & Francis
Abstract The trisaccharide, 2′-fucosyllactose (Fucα1-2Galβ1-4Glc; 2′-FL), is the most
abundant oligosaccharide in human milk. It has numerous significant biological properties …
abundant oligosaccharide in human milk. It has numerous significant biological properties …
Strategies for enhancing microbial production of 2′-fucosyllactose, the most abundant human milk oligosaccharide
Y Liu, Y Zhu, H Wang, L Wan, W Zhang… - Journal of Agricultural …, 2022 - ACS Publications
Human milk oligosaccharides (HMOs), a group of structurally diverse unconjugated glycans
in breast milk, act as important prebiotics and have plenty of unique health effects for …
in breast milk, act as important prebiotics and have plenty of unique health effects for …
Combinatorial metabolic engineering and tolerance evolving of Escherichia coli for high production of 2′-fucosyllactose
X Sun, Z Peng, C Li, Y Zheng, J Zong, F Lu, Y Li… - Bioresource …, 2023 - Elsevier
Fucosyllactose (2′-FL) is an important functional ingredient of advanced infant formula.
Here, Escherichia coli MG1655 was engineered for achieving high 2′-FL production. The …
Here, Escherichia coli MG1655 was engineered for achieving high 2′-FL production. The …
Construction of Escherichia coli strains with chromosomally integrated expression cassettes for the synthesis of 2′-fucosyllactose
F Baumgärtner, L Seitz, GA Sprenger… - Microbial Cell …, 2013 - Springer
Abstract Background The trisaccharide 2′-fucosyllactose (2′-FL) is one of the most
abundant oligosaccharides found in human milk. Due to its prebiotic and anti-infective …
abundant oligosaccharides found in human milk. Due to its prebiotic and anti-infective …
Biotechnological production of fucosylated human milk oligosaccharides: Prokaryotic fucosyltransferases and their use in biocatalytic cascades or whole cell …
B Petschacher, B Nidetzky - Journal of Biotechnology, 2016 - Elsevier
Human milk oligosaccharides (HMOs) constitute a class of complex carbohydrates unique to
mother's milk and are strongly correlated to the health benefits of breastfeeding in infants …
mother's milk and are strongly correlated to the health benefits of breastfeeding in infants …
Whole cell biosynthesis of a functional oligosaccharide, 2′-fucosyllactose, using engineered Escherichia coli
WH Lee, P Pathanibul, J Quarterman, JH Jo… - Microbial Cell …, 2012 - Springer
Abstract Background 2'-Fucosyllactose (2-FL) is a functional oligosaccharide present in
human milk which protects against the infection of enteric pathogens. Because 2-FL can be …
human milk which protects against the infection of enteric pathogens. Because 2-FL can be …
Enhanced production of 2′-fucosyllactose in engineered Escherichia coli BL21star (DE3) by modulation of lactose metabolism and fucosyltransferase
Fucosyllactose (2-FL) is one of most abundant functional oligosaccharides in human milk,
which is involved in many biological functions for human health. To date, most microbial …
which is involved in many biological functions for human health. To date, most microbial …
Biotechnological production of human milk oligosaccharides
Human milk contains a large variety of oligosaccharides (HMOs) that have the potential to
modulate the gut flora, affect different gastrointestinal functions, and influence inflammatory …
modulate the gut flora, affect different gastrointestinal functions, and influence inflammatory …
Engineered microbial routes for human milk oligosaccharides synthesis
Human milk oligosaccharides (HMOs) are one of the important ingredients in human milk,
which have attracted great interest due to their beneficial effect on the health of newborns …
which have attracted great interest due to their beneficial effect on the health of newborns …