Choose your own adventure: A comprehensive database of reactions catalyzed by cytochrome P450 BM3 variants

DJ Fansher, JN Besna, A Fendri, JN Pelletier - ACS catalysis, 2024 - ACS Publications
Cytochrome P450 BM3 monooxygenase is the topic of extensive research as many
researchers have evolved this enzyme to generate a variety of products. However, the …

Engineered microbes as effective tools for the remediation of polyaromatic aromatic hydrocarbons and heavy metals

P Sharma, A Bano, SP Singh, S Sharma, C **a… - Chemosphere, 2022 - Elsevier
Heavy metals (HMs) and polycyclic aromatic hydrocarbons (PAHs) have become a major
concern to human health and the environment due to rapid industrialization and …

Constructing Protein-Scaffolded Multienzyme Assembly Enhances the Coupling Efficiency of the P450 System for Efficient Daidzein Biosynthesis from (2S) …

Z Wang, Y Dai, F Azi, Z Wang, W Xu… - Journal of agricultural …, 2024 - ACS Publications
Daidzein is a major isoflavone compound with an immense pharmaceutical value. This
study applied a novel P450 CYP82D26 which can biosynthesize daidzein from (2 S) …

Synthetically engineered microbial scavengers for enhanced bioremediation

KM Tran, HM Lee, TD Thai, J Shen, S Eyun… - Journal of hazardous …, 2021 - Elsevier
Microbial bioremediation has gained attention as a cheap, efficient, and sustainable
technology to manage the increasing environmental pollution. Since microorganisms in …

[HTML][HTML] Growth-coupled enzyme engineering through manipulation of redox cofactor regeneration

JR Nielsen, RA Weusthuis, WE Huang - Biotechnology Advances, 2023 - Elsevier
Enzymes need to be efficient, robust, and highly specific for their effective use in commercial
bioproduction. These properties can be introduced using various enzyme engineering …

Current trend of polycyclic aromatic hydrocarbon bioremediation: Mechanism, artificial mixed microbial strategy, machine learning, ground application, cost and policy …

D Baskaran, HS Byun - Chemical Engineering Journal, 2024 - Elsevier
Global market growth has increased demand for non-renewable fossil fuels, posing a threat
to the environment and human health. Environmentalists seek eco-friendly ways to treat …

Directed evolution of phosphite dehydrogenase to cycle noncanonical redox cofactors via universal growth selection platform

L Zhang, E King, WB Black, CM Heckmann… - Nature …, 2022 - nature.com
Noncanonical redox cofactors are attractive low-cost alternatives to nicotinamide adenine
dinucleotide (phosphate)(NAD (P)+) in biotransformation. However, engineering enzymes to …

The molecular basis and enzyme engineering strategies for improvement of coupling efficiency in cytochrome P450s

S Meng, Y Ji, LL Zhu, GV Dhoke, MD Davari… - Biotechnology …, 2022 - Elsevier
Cytochrome P450s are heme-thiolate enzymes that have been broadly applied in
pharmaceutical and biosynthesis because of their efficient oxidation at inert carbons …

Growth‐Coupled Evolutionary Pressure Improving Epimerases for D‐Allulose Biosynthesis Using a Biosensor‐Assisted In Vivo Selection Platform

C Li, X Gao, H Li, T Wang, F Lu, HM Qin - Advanced Science, 2024 - Wiley Online Library
Fast screening strategies that enable high‐throughput evaluation and identification of
desired variants from diversified enzyme libraries are crucial to tailoring biocatalysts for the …

Orthogonal glycolytic pathway enables directed evolution of noncanonical cofactor oxidase

E King, S Maxel, Y Zhang, KC Kenney, Y Cui… - Nature …, 2022 - nature.com
Noncanonical cofactor biomimetics (NCBs) such as nicotinamide mononucleotide (NMN+)
provide enhanced scalability for biomanufacturing. However, engineering enzymes to …