Mechanism-based design of efficient PET hydrolases

R Wei, G von Haugwitz, L Pfaff, J Mican… - ACS …, 2022 - ACS Publications
Polyethylene terephthalate (PET) is the most widespread synthetic polyester, having been
utilized in textile fibers and packaging materials for beverages and food, contributing …

Mechanism and characterization of microplastic aging process: A review

Q Lu, Y Zhou, Q Sui, Y Zhou - Frontiers of Environmental Science & …, 2023 - Springer
With the increasing production of petroleum-based plastics, the problem of environmental
pollution caused by plastics has aroused widespread concern. Microplastics, which are …

Multiple substrate binding mode-guided engineering of a thermophilic PET hydrolase

L Pfaff, J Gao, Z Li, A Jäckering, G Weber… - ACS …, 2022 - ACS Publications
Thermophilic polyester hydrolases (PES-H) have recently enabled biocatalytic recycling of
the mass-produced synthetic polyester polyethylene terephthalate (PET), which has found …

Structural insights into (tere) phthalate-ester hydrolysis by a carboxylesterase and its role in promoting PET depolymerization

G von Haugwitz, X Han, L Pfaff, Q Li, H Wei, J Gao… - ACS …, 2022 - ACS Publications
TfCa, a promiscuous carboxylesterase from Thermobifida fusca, was found to hydrolyze
polyethylene terephthalate (PET) degradation intermediates such as bis (2-hydroxyethyl) …

Engineering and evaluation of thermostable IsPETase variants for PET degradation

S Brott, L Pfaff, J Schuricht, JN Schwarz… - Engineering in life …, 2022 - Wiley Online Library
Polyethylene terephthalate (PET) is a mass‐produced petroleum‐based synthetic polymer.
Enzymatic PET degradation using, for example, Ideonella sakaiensis PETase (IsPETase) …

A comprehensive review on enzymatic biodegradation of polyethylene terephthalate

J Qiu, Y Chen, L Zhang, J Wu, X Zeng, X Shi, L Liu… - Environmental …, 2024 - Elsevier
Polyethylene terephthalate (PET) is a polymer synthesized via the dehydration and
condensation reaction between ethylene glycol and terephthalic acid. PET has emerged as …

Nano/microplastics capture and degradation by autonomous nano/microrobots: a perspective

M Urso, M Pumera - Advanced Functional Materials, 2022 - Wiley Online Library
The growing use of plastic materials has led to the continuous accumulation of wastes in
marine environments, which fragment into hazardous micro‐and nanoplastics. These plastic …

Sub-micro-and nano-sized polyethylene terephthalate deconstruction with engineered protein nanopores

A Robles-Martín, R Amigot-Sánchez… - Nature Catalysis, 2023 - nature.com
The identification or design of biocatalysts to mitigate the accumulation of plastics, including
sub-micro-and nano-sized polyethylene terephthalate (nPET), is becoming a global …

Positive charge introduction on the surface of thermostabilized PET hydrolase facilitates PET binding and degradation

A Nakamura, N Kobayashi, N Koga, R Iino - ACS Catalysis, 2021 - ACS Publications
A thermostable enzyme PET2, found in a metagenome library, has been engineered to
improve its hydrolytic activity against polyethylene terephthalate (PET). The PET2 wild-type …

Nanoplastics: detection and impacts in aquatic environments–a review

N Kokilathasan, M Dittrich - Science of the Total Environment, 2022 - Elsevier
The rise in the global production of plastics has led to severe concerns about the impacts of
plastics in aquatic environments. Although plastic materials degrade over extreme long …