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Probing protein folding and conformational transitions with fluorescence
Fluorescence arguably constitutes the most widely used experimental approach in the field
of protein folding. Hence, any review of the use of fluorescence to probe protein stability and …
of protein folding. Hence, any review of the use of fluorescence to probe protein stability and …
Fluorescence correlation spectroscopy: past, present, future
In recent years fluorescence correlation spectroscopy (FCS) has become a routine method
for determining diffusion coefficients, chemical rate constants, molecular concentrations …
for determining diffusion coefficients, chemical rate constants, molecular concentrations …
[كتاب][B] Principles of fluorescence spectroscopy
JR Lakowicz - 2006 - Springer
The success of fluorescence experiments requires attention to experimental details and an
understanding of the instrumentation. There are also many potential artifacts that can distort …
understanding of the instrumentation. There are also many potential artifacts that can distort …
Inert and seed-competent tau monomers suggest structural origins of aggregation
Tauopathies feature progressive accumulation of tau amyloids. Pathology may begin when
these amplify from a protein template, or seed, whose structure is unknown. We have …
these amplify from a protein template, or seed, whose structure is unknown. We have …
Tau trimers are the minimal propagation unit spontaneously internalized to seed intracellular aggregation
Tau amyloid assemblies propagate aggregation from the outside to the inside of a cell,
which may mediate progression of the tauopathies. The critical size of Tau assemblies, or" …
which may mediate progression of the tauopathies. The critical size of Tau assemblies, or" …
Bioorthogonal labeling with tetrazine-dyes for super-resolution microscopy
Genetic code expansion (GCE) technology allows the specific incorporation of
functionalized noncanonical amino acids (ncAAs) into proteins. Here, we investigated the …
functionalized noncanonical amino acids (ncAAs) into proteins. Here, we investigated the …
Single-molecule biophysics: at the interface of biology, physics and chemistry
Single-molecule methods have matured into powerful and popular tools to probe the
complex behaviour of biological molecules, due to their unique abilities to probe molecular …
complex behaviour of biological molecules, due to their unique abilities to probe molecular …
Shot-noise limited single-molecule FRET histograms: comparison between theory and experiments
We describe a simple approach and present a straightforward numerical algorithm to
compute the best fit shot-noise limited proximity ratio histogram (PRH) in single-molecule …
compute the best fit shot-noise limited proximity ratio histogram (PRH) in single-molecule …
Single-molecule studies of protein folding
Although protein-folding studies began several decades ago, it is only recently that the tools
to analyze protein folding at the single-molecule level have been developed. Advances in …
to analyze protein folding at the single-molecule level have been developed. Advances in …
A natively unfolded yeast prion monomer adopts an ensemble of collapsed and rapidly fluctuating structures
The yeast prion protein Sup35 is a translation termination factor, whose activity is modulated
by sequestration into a self-perpetuating amyloid. The prion-determining domain, NM …
by sequestration into a self-perpetuating amyloid. The prion-determining domain, NM …