[HTML][HTML] Far-Red Fluorescent Proteins: Tools for Advancing In Vivo Imaging

A Shang, S Shao, L Zhao, B Liu - Biosensors, 2024 - mdpi.com
Far-red fluorescent proteins (FPs) have emerged as indispensable tools in in vivo imaging,
playing a pivotal role in elucidating fundamental mechanisms and addressing application …

Influence of fluorescence lifetime selections and conformational flexibility on brightness of fusionRed variants

S Mukherjee, N Douglas, R Jimenez - The Journal of Physical …, 2024 - ACS Publications
Fluorescent proteins (FPs) for bioimaging are typically developed by screening mutant
libraries for clones with improved photophysical properties. This approach has resulted in …

Conformational dynamics of mCherry variants: a link between side-chain motions and fluorescence brightness

S Mukherjee, P Manna, N Douglas… - The Journal of …, 2022 - ACS Publications
The 3-fold higher brightness of the recently developed mCherry-XL red fluorescent protein
(FP) compared to its progenitor, mCherry, is due to a significant decrease in the nonradiative …

The luminescence hypothesis of olfaction

K Willeford - Sensors, 2023 - mdpi.com
A new hypothesis for the mechanism of olfaction is presented. It begins with an odorant
molecule binding to an olfactory receptor. This is followed by the quantum biology event of …

The two key substitutions in the chromophore environment of mKate2 to produce an enhanced FusionRed-like red fluorescent protein

DA Ruchkin, AS Gavrikov, DV Kolesov… - bioRxiv, 2023 - biorxiv.org
Red fluorescent proteins (RFPs) can be considered as a probe of choice for living tissue
microscopy and whole-body imaging. When choosing a specific RFP variant, the priority …

[PDF][PDF] Supporting Information: Single-Molecule Stoichiometry of Supramolecular Complexes

A McLean, RL Sala, BW Longbottom, AR Carr… - repository.cam.ac.uk
Unless otherwise specified, all materials were purchased from commercial suppliers as
detailed below and were used without further purification. 9, 10-dibromoanthracene (Sigma …

Spectroscopic Evaluation of Excited State Depopulation in Red Fluorescent Proteins Developed Using Fluorescence Lifetime Selections

S Mukherjee - 2022 - search.proquest.com
Fluorescent Proteins are intriguing molecules that are ubiquitous tools for biological
research. This thesis investigates the relationship between engineering improved …

[PDF][PDF] Supplementary Information-Directed evolution of a bright variant of mCherry: Suppression of non-radiative decay by fluorescence lifetime selections

S Mukherjee, P Manna, ST Hung, F Vietmeyer, P Friis… - chemrxiv.org
1 Mutagenesis protocols 2 Mutagenesis and directed evolution of mCherry 3 Sequence
alignment of the RFP variants 4 Additional in cellulo assessments 5 Additional in vitro …