Capillary forces generated by biomolecular condensates

B Gouveia, Y Kim, JW Shaevitz, S Petry, HA Stone… - Nature, 2022 - nature.com
Liquid–liquid phase separation and related phase transitions have emerged as generic
mechanisms in living cells for the formation of membraneless compartments or biomolecular …

Theoretical and data-driven approaches for biomolecular condensates

KL Saar, D Qian, LL Good, AS Morgunov… - Chemical …, 2023 - ACS Publications
Biomolecular condensation processes are increasingly recognized as a fundamental
mechanism that living cells use to organize biomolecules in time and space. These …

Aging can transform single-component protein condensates into multiphase architectures

A Garaizar, JR Espinosa, JA Joseph… - Proceedings of the …, 2022 - National Acad Sciences
Phase-separated biomolecular condensates that contain multiple coexisting phases are
widespread in vitro and in cells. Multiphase condensates emerge readily within …

Fluorescent protein lifetimes report densities and phases of nuclear condensates during embryonic stem-cell differentiation

K Joron, JO Viegas, L Haas-Neill, S Bier, P Drori… - Nature …, 2023 - nature.com
Fluorescent proteins (FP) are frequently used for studying proteins inside cells. In advanced
fluorescence microscopy, FPs can report on additional intracellular variables. One variable …

Construction of multiphasic membraneless organelles towards spontaneous spatial segregation and directional flow of biochemical reactions

F Zhorabek, MS Abesekara, J Liu, X Dai, J Huang… - Chemical …, 2023 - pubs.rsc.org
Many intracellular membraneless organelles (MLOs) appear to adapt a hierarchical
multicompartment organization for efficient coordination of highly complex reaction …

Thermodynamic origins of two-component multiphase condensates of proteins

PY Chew, JA Joseph, R Collepardo-Guevara… - Chemical …, 2023 - pubs.rsc.org
Intracellular condensates are highly multi-component systems in which complex phase
behaviour can ensue, including the formation of architectures comprising multiple …

Protein structural transitions critically transform the network connectivity and viscoelasticity of RNA-binding protein condensates but RNA can prevent it

AR Tejedor, I Sanchez-Burgos… - Nature …, 2022 - nature.com
Biomolecular condensates, some of which are liquid-like during health, can age over time
becoming gel-like pathological systems. One potential source of loss of liquid-like properties …

Amphiphilic proteins coassemble into multiphasic condensates and act as biomolecular surfactants

FM Kelley, B Favetta, RM Regy… - Proceedings of the …, 2021 - National Acad Sciences
Cells contain membraneless compartments that assemble due to liquid–liquid phase
separation, including biomolecular condensates with complex morphologies. For instance …

Surface electrostatics govern the emulsion stability of biomolecular condensates

TJ Welsh, G Krainer, JR Espinosa, JA Joseph… - Nano …, 2022 - ACS Publications
Liquid–liquid phase separation underlies the formation of biological condensates.
Physically, such systems are microemulsions that in general have a propensity to fuse and …

[HTML][HTML] Salt dependent phase behavior of intrinsically disordered proteins from a coarse-grained model with explicit water and ions

A Garaizar, JR Espinosa - The Journal of chemical physics, 2021 - pubs.aip.org
Multivalent proteins and nucleic acids can self-assemble into biomolecular condensates that
contribute to compartmentalize the cell interior. Computer simulations offer a unique view to …