The function that cumulus cells play during oocyte maturation ie, in kee** the oocyte
under arrest, in participating in the induction of meiotic resumption and in supporting …

As a result of research in the 1980s on in vitro maturation, sperm capacitation, and in vitro
fertilization, the bovine is now one of the important models for development. Further, the …

The objectives of these experiments were to characterize separation of frozen-thawed
bovine spermatozoa on a Percoll gradient and then to compare sperm separation by either a …

Epidermal growth factor (EGF) has been shown to have a positive effect during in vitro
maturation (IVM) and has been reported in follicular fluid at levels capable of stimulating …

Several contemporary micromanipulation techniques, such as sperm microinjection, nuclear
transfer, and gene transfer by pronuclear injection, require removal of cumulus cells from …

Two experiments were conducted to assess the effects of environmental temperature and
humidity on the quality and developmental capabilities of bovine oocytes. In Experiment 1 …

Various factors, such as quality of the oocyte, oxygen tension, embryo density, and kind of
energy substrate during in vitro production of embryos may affect the rate of preimplantation …

The transition from the maternal to embryonic control of early embryonic development (MET)
in mammals is not fully understood. The objective of this study was to determine the amount …

Bovine oocytes matured in vitro were fertilized in high proportions (92% of matured oocytes)
by sperm capacitated with Ca ionophore A23187. Eight percent of inseminated oocytes that …

Embryo transfer has become the most powerful tool animal breeders have acquired since
artificial insemination. Several countries, including Canada, have incorporated multiple …