DNA supercoiling and transcription in bacteria: a two-way street
CJ Dorman - BMC molecular and cell biology, 2019 - Springer
Background The processes of DNA supercoiling and transcription are interdependent
because the movement of a transcription elongation complex simultaneously induces under …
because the movement of a transcription elongation complex simultaneously induces under …
Real-time imaging of DNA loop extrusion by condensin
It has been hypothesized that SMC protein complexes such as condensin and cohesin
spatially organize chromosomes by extruding DNA into large loops. We directly visualized …
spatially organize chromosomes by extruding DNA into large loops. We directly visualized …
DNA packaging by molecular motors: from bacteriophage to human chromosomes
Dense packaging of genomic DNA is crucial for organismal survival, as DNA length always
far exceeds the dimensions of the cells that contain it. Organisms, therefore, use …
far exceeds the dimensions of the cells that contain it. Organisms, therefore, use …
Measuring DNA mechanics on the genome scale
Mechanical deformations of DNA such as bending are ubiquitous and have been implicated
in diverse cellular functions. However, the lack of high-throughput tools to measure the …
in diverse cellular functions. However, the lack of high-throughput tools to measure the …
Dynamic ParB–DNA interactions initiate and maintain a partition condensate for bacterial chromosome segregation
In most bacteria, chromosome segregation is driven by the ParAB S system where the
CTPase protein ParB loads at the parS site to trigger the formation of a large partition …
CTPase protein ParB loads at the parS site to trigger the formation of a large partition …
Single-molecule visualization of twin-supercoiled domains generated during transcription
Transcription-coupled supercoiling of DNA is a key factor in chromosome compaction and
the regulation of genetic processes in all domains of life. It has become common knowledge …
the regulation of genetic processes in all domains of life. It has become common knowledge …
Base-pair resolution analysis of the effect of supercoiling on DNA flexibility and major groove recognition by triplex-forming oligonucleotides
In the cell, DNA is arranged into highly-organised and topologically-constrained
(supercoiled) structures. It remains unclear how this supercoiling affects the detailed double …
(supercoiled) structures. It remains unclear how this supercoiling affects the detailed double …
Supercoiling-mediated feedback rapidly couples and tunes transcription
CP Johnstone, KE Galloway - Cell reports, 2022 - cell.com
Transcription induces a wave of DNA supercoiling, altering the binding affinity of RNA
polymerases and resha** the biochemical landscape of gene regulation. As supercoiling …
polymerases and resha** the biochemical landscape of gene regulation. As supercoiling …
Loop-extruding Smc5/6 organizes transcription-induced positive DNA supercoils
The structural maintenance of chromosomes (SMC) protein complexes—cohesin,
condensin, and the Smc5/6 complex (Smc5/6)—are essential for chromosome function. At …
condensin, and the Smc5/6 complex (Smc5/6)—are essential for chromosome function. At …
Chiral systems made from DNA
The very chemical structure of DNA that enables biological heredity and evolution has non‐
trivial implications for the self‐organization of DNA molecules into larger assemblies and …
trivial implications for the self‐organization of DNA molecules into larger assemblies and …