Microbial communities might include distinct lineages of closely related organisms that
complicate metagenomic assembly and prevent the generation of complete metagenome …

Microbiology has long studied the ways in which subtle genetic differences between closely
related microbial strains can have profound impacts on their phenotypes and those of their …

Metagenomics has revolutionized our understanding of microbial communities, offering
unprecedented insights into their genetic and functional diversity across Earth's diverse …

Monitoring SARS-CoV-2 in wastewater has shown to be an effective tool for epidemiological
surveillance. More specifically, RNA levels determined with RT-qPCR have been shown to …

Next-generation sequencing–based metagenomics has enabled to identify microorganisms
in characteristic habitats without the need for lengthy cultivation. Importantly, clinically …

The advent of long and accurate “HiFi” reads has greatly improved our ability to generate
complete metagenome-assembled genomes (MAGs), enabling “complete metagenomics” …

Bacterial species in microbial communities are often represented by mixtures of strains,
distinguished by small variations in their genomes. Short-read approaches can be used to …

Microbial communities are usually highly diverse and often involve multiple strains from the
participating species due to the rapid evolution of microorganisms. In such a complex …

Background Metagenomic assembly using high-throughput sequencing data is a powerful
method to construct microbial genomes in environmental samples without cultivation …

Real-world evaluations of metagenomic reconstructions are challenged by distinguishing
reconstruction artifacts from genes and proteins present in situ. Here, we evaluate short-read …