Excited state reactions in fluorescent proteins

SR Meech - Chemical Society Reviews, 2009 - pubs.rsc.org
The green fluorescent protein is a key technology in bioimaging. In this critical review, we
consider how its various applications can be tailored from knowledge of the excited state …

Chromophore transformations in red fluorescent proteins

FV Subach, VV Verkhusha - Chemical reviews, 2012 - ACS Publications
1. INTRODUCTION The discovery of Anthozoa homologues of the green fluorescent protein
(GFP) from jellyfish Aequorea victoria, which emit not only green but also yellow, orange …

Genetically encoded calcium indicators for multi-color neural activity imaging and combination with optogenetics

J Akerboom, N Carreras Calderón, L Tian… - Frontiers in molecular …, 2013 - frontiersin.org
Genetically encoded calcium indicators (GECIs) are powerful tools for systems
neuroscience. Here we describe red, single-wavelength GECIs,“RCaMPs,” engineered from …

GFP family: structural insights into spectral tuning

AA Pakhomov, VI Martynov - Chemistry & biology, 2008 - cell.com
Proteins homologous to green fluorescent protein (GFP) span most of the visible spectrum,
offering indispensable tools for live cell imaging. Structural transformations, such as …

Increased mitochondrial free Ca2+ during ischemia is suppressed, but not eliminated by, germline deletion of the mitochondrial Ca2+ uniporter

CE Petersen, J Sun, K Silva, A Kosmach, RS Balaban… - Cell reports, 2023 - cell.com
Mitochondrial Ca 2+ overload is proposed to regulate cell death via opening of the
mitochondrial permeability transition pore. It is hypothesized that inhibition of the …

Dark states in monomeric red fluorescent proteins studied by fluorescence correlation and single molecule spectroscopy

J Hendrix, C Flors, P Dedecker, J Hofkens… - Biophysical journal, 2008 - cell.com
Monomeric red fluorescent proteins (mRFPs) have become indispensable tools for studying
protein dynamics, interactions and functions in the cellular environment. Their emission …

Bond selection in the photoisomerization reaction of anionic green fluorescent protein and kindling fluorescent protein chromophore models

S Olsen, SC Smith - Journal of the American Chemical Society, 2008 - ACS Publications
The chromophores of the most widely known fluorescent proteins (FPs) are derivatives of a
core p-hydroxybenzylidene-imidazolinon-5-one (HBI) motif, which usually occurs as a …

The role of host pigments in coral photobiology

G Ferreira, E Bollati, M Kühl - Frontiers in Marine Science, 2023 - frontiersin.org
Corals have the ability to synthesize various pigments, responsible for their characteristic
vivid coloration. Most coral host pigments are green fluorescent protein (GFP)-like pigments …

Mutants of the flavoprotein iLOV as prospective red-shifted fluorescent markers

MG Khrenova, YI Meteleshko… - The Journal of Physical …, 2017 - ACS Publications
We report on novel variants of the flavin-based fluorescent protein iLOV with absorption and
emission optical bands shifted to the longer wavelengths relative to their precursor. First …

Ultramarine, a chromoprotein acceptor for Förster resonance energy transfer

A Pettikiriarachchi, L Gong, MA Perugini, RJ Devenish… - PloS one, 2012 - journals.plos.org
We have engineered a monomeric blue non-fluorescent chromoprotein called Ultramarine
(fluorescence quantum yield, 0.001; ε 585 nm, 64,000 M− 1. cm− 1) for use as a Förster …