Almost all proteins contain charged amino acids. While the function in catalysis or binding of
individual charges in the active site can often be identified, it is less clear how to assign …

Native mass spectrometry is a rapidly emerging technique for fast and sensitive structural
analysis of protein constructs, maintaining the protein higher order structure. The coupling …

Outlining the fundamental principles by which all interactions occur, this reference focuses
on harnessing the biochemistry of bioorganic compounds in order to separate them …

The adsorption of proteins with net positive charges (p I> pH) on the walls of fused-silica
capillaries is a common problem in the analysis of proteins by capillary electrophoresis. This …

Electrostatic forces play a key role in mediating interactions between proteins. However,
gaining quantitative insights into the complex effects of electrostatics on protein behavior …

Affinity capillary electrophoresis (ACE) is a technique that is used to measure the binding
affinity of receptors to neutral and charged ligands. ACE experiments are based on …

Cytometry deals with the analysis of the composition of single cells. Flow and image
cytometry employ antibody-based stains to characterize a handful of components in single …

Recent studies of globular protein solutions have uniformly adopted a colloidal view of
proteins as particles, a perspective that neglects the polymeric primary structure of these …

Affinity capillary electrophoresis (ACE) is a new procedure forstudying protein-ligand
interactions. 1-9 Its potential applications include develo** tight-binding drug candidates …

In this work, we used direct measurements with the surface force apparatus to determine the
pH-dependent electrostatic charge density of a single binding face of streptavidin. Mean …