The ideal scenario in most applications of microbial diagnostics is that only viable cells are
detected. Bacteria were traditionally considered viable when they could be cultured …

The increase in foodborne outbreaks highlights the need for rapid, sensitive and specific
methods for food safety monitoring, enabling specific detection and quantification of viable …

Quantitative real-time PCR (qPCR) has been widely used in recent environmental microbial
ecology studies as a tool for detecting and quantifying microorganisms of interest, which …

Thanks to the achievements in sanitation, hygiene practices, and antibiotics, we have
considerably improved in our ongoing battle against pathogenic bacteria. However, with our …

Foodborne outbreaks are a serious public health and food safety concern worldwide. There
is a great demand for rapid, sensitive, specific, and accurate methods to detect microbial …

Environmental DNA (eDNA) analysis allows cost-effective and non-destructive
biomonitoring with a high detection sensitivity in terrestrial and aquatic environments …

Opportunistic premise (ie, building) plumbing pathogens (OPPPs, eg, Legionella
pneumophila, Mycobacterium avium complex, Pseudomonas aeruginosa, Acanthamoeba …

Background Leptospirosis is an important re-emerging infectious disease that affects
humans worldwide. Infection occurs from indirect environment-mediated exposure to …

Background High-throughput sequencing provides a powerful window into the structural and
functional profiling of microbial communities, but it is unable to characterize only the viable …

Major waterborne (enteric) pathogens are relatively well understood and treatment controls
are effective when well managed. However, water-based, saprozoic pathogens that grow …