Data analysis guidelines for single-cell RNA-seq in biomedical studies and clinical applications
M Su, T Pan, QZ Chen, WW Zhou, Y Gong, G Xu… - Military Medical …, 2022 - Springer
The application of single-cell RNA sequencing (scRNA-seq) in biomedical research has
advanced our understanding of the pathogenesis of disease and provided valuable insights …
advanced our understanding of the pathogenesis of disease and provided valuable insights …
Context-specific regulation and function of mRNA alternative polyadenylation
Alternative cleavage and polyadenylation (APA) is a widespread mechanism to generate
mRNA isoforms with alternative 3′ untranslated regions (UTRs). The expression of …
mRNA isoforms with alternative 3′ untranslated regions (UTRs). The expression of …
[HTML][HTML] Molecular topography of an entire nervous system
We have produced gene expression profiles of all 302 neurons of the C. elegans nervous
system that match the single-cell resolution of its anatomy and wiring diagram. Our results …
system that match the single-cell resolution of its anatomy and wiring diagram. Our results …
[HTML][HTML] Hif-1a suppresses ROS-induced proliferation of cardiac fibroblasts following myocardial infarction
We report that cardiac fibroblasts (CFs) and mesenchymal progenitors are more hypoxic
than other cardiac interstitial populations, express more hypoxia-inducible factor 1α (HIF …
than other cardiac interstitial populations, express more hypoxia-inducible factor 1α (HIF …
Multiplexed single-cell characterization of alternative polyadenylation regulators
Most mammalian genes have multiple polyA sites, representing a substantial source of
transcript diversity regulated by the cleavage and polyadenylation (CPA) machinery. To …
transcript diversity regulated by the cleavage and polyadenylation (CPA) machinery. To …
Integration map** of cardiac fibroblast single-cell transcriptomes elucidates cellular principles of fibrosis in diverse pathologies
Single-cell technology has allowed researchers to probe tissue complexity and dynamics at
unprecedented depth in health and disease. However, the generation of high-dimensionality …
unprecedented depth in health and disease. However, the generation of high-dimensionality …
BamQuery: a proteogenomic tool to explore the immunopeptidome and prioritize actionable tumor antigens
MHC-I-associated peptides deriving from non-coding genomic regions and mutations can
generate tumor-specific antigens, including neoantigens. Quantifying tumor-specific …
generate tumor-specific antigens, including neoantigens. Quantifying tumor-specific …
Studying stochastic systems biology of the cell with single-cell genomics data
Recent experimental developments in genome-wide RNA quantification hold considerable
promise for systems biology. However, rigorously probing the biology of living cells requires …
promise for systems biology. However, rigorously probing the biology of living cells requires …
Characterization of transcript enrichment and detection bias in single-nucleus RNA-seq for map** of distinct human adipocyte lineages
Single-cell RNA sequencing (scRNA-seq) enables molecular characterization of complex
biological tissues at high resolution. The requirement of single-cell extraction, however …
biological tissues at high resolution. The requirement of single-cell extraction, however …
On the function and relevance of alternative 3′‐UTRs in gene expression regulation
Messanger RNA (mRNA) isoforms with alternative 3′‐untranslated regions (3′‐UTRs) are
produced by alternative polyadenylation (APA), which occurs during transcription in most …
produced by alternative polyadenylation (APA), which occurs during transcription in most …