The recent explosion in the diversity of available fluorescent proteins (FPs),,,,,,,,,,,,,,,
promises a wide variety of new tools for biological imaging. With no unified standard for …

Green fluorescent protein (GFP) from the jellyfish Aequorea victoria and its homologs from
diverse marine animals are widely used as universal genetically encoded fluorescent labels …

We report the engineering of mScarlet, a truly monomeric red fluorescent protein with record
brightness, quantum yield (70%) and fluorescence lifetime (3.9 ns). We developed mScarlet …

Biomolecular condensates concentrate macromolecules into discrete cellular foci without an
encapsulating membrane. Condensates are often presumed to increase enzymatic reaction …

Study of the nematode Caenorhabditis elegans has provided important insights in a wide
range of fields in biology. The ability to precisely modify genomes is critical to fully realize …

Cellular signaling networks are the foundation which determines the fate and function of
cells as they respond to various cues and stimuli. The discovery of fluorescent proteins over …

Förster or fluorescence resonance energy transfer (FRET) technology and genetically
encoded FRET biosensors provide a powerful tool for visualizing signaling molecules in live …

Fluorescence resonance energy transfer (FRET) is a nonradiative process whereby an
excited state donor D (usually a fluorophore) transfers energy to a proximal ground state …

Genetically encoded calcium indicators (GECIs) are powerful tools for systems
neuroscience. Here we describe red, single-wavelength GECIs,“RCaMPs,” engineered from …

The advent of fluorescent proteins (FPs) for genetic labeling of molecules and cells has
revolutionized fluorescence microscopy. Genetic manipulations have created a vast array of …