Genome engineering for crop improvement and future agriculture

C Gao - Cell, 2021 - cell.com
Feeding the ever-growing population is a major challenge, especially in light of rapidly
changing climate conditions. Genome editing is set to revolutionize plant breeding and …

Recent advances in crop transformation technologies

Z Chen, JM Debernardi, J Dubcovsky, A Gallavotti - Nature plants, 2022 - nature.com
Agriculture is experiencing a technological inflection point in its history, while also facing
unprecedented challenges posed by human population growth and global climate changes …

Cut–dip–budding delivery system enables genetic modifications in plants without tissue culture

X Cao, H **e, M Song, J Lu, P Ma, B Huang, M Wang… - The Innovation, 2023 - cell.com
Of the more than 370 000 species of higher plants in nature, fewer than 0.1% can be
genetically modified due to limitations of the current gene delivery systems. Even for those …

Leaf transformation for efficient random integration and targeted genome modification in maize and sorghum

N Wang, L Ryan, N Sardesai, E Wu, B Lenderts… - Nature Plants, 2023 - nature.com
Transformation in grass species has traditionally relied on immature embryos and has
therefore been limited to a few major Poaceae crops. Other transformation explants …

A GRF–GIF chimeric protein improves the regeneration efficiency of transgenic plants

JM Debernardi, DM Tricoli, MF Ercoli, S Hayta… - Nature …, 2020 - nature.com
The potential of genome editing to improve the agronomic performance of crops is often
limited by low plant regeneration efficiencies and few transformable genotypes. Here, we …

[HTML][HTML] CRISPR/Cas-mediated plant genome editing: outstanding challenges a decade after implementation

T Cardi, J Murovec, A Bakhsh, J Boniecka… - Trends in Plant …, 2023 - cell.com
The discovery of the CRISPR/Cas genome-editing system has revolutionized our
understanding of the plant genome. CRISPR/Cas has been used for over a decade to …

Highly efficient heritable genome editing in wheat using an RNA virus and bypassing tissue culture

T Li, J Hu, Y Sun, B Li, D Zhang, W Li, J Liu, D Li… - Molecular Plant, 2021 - cell.com
Genome editing provides novel strategies for improving plant traits but mostly relies on
conventional plant genetic transformation and regeneration procedures, which can be …

Establishment of an Agrobacterium‐mediated genetic transformation and CRISPR/Cas9‐mediated targeted mutagenesis in Hemp (Cannabis Sativa L.)

X Zhang, G Xu, C Cheng, L Lei, J Sun… - Plant biotechnology …, 2021 - Wiley Online Library
Hemp (Cannabis sativa L.) is an annual and typically dioecious crop. Due to the therapeutic
potential for human diseases, phytocannabinoids as a medical therapy is getting more …

Agrobacterium expressing a type III secretion system delivers Pseudomonas effectors into plant cells to enhance transformation

V Raman, CM Rojas, B Vasudevan, K Dunning… - Nature …, 2022 - nature.com
Agrobacterium-mediated plant transformation (AMT) is the basis of modern-day plant
biotechnology. One major drawback of this technology is the recalcitrance of many plant …

Strategies for genotype-flexible plant transformation

K Lee, K Wang - Current Opinion in Biotechnology, 2023 - Elsevier
Recent advances in the genome-editing tools have demonstrated a great potential for
accelerating functional genomics and crop trait improvements, but the low efficiency and …