In vitro plant tissue culture: means for production of biological active compounds

CA Espinosa-Leal, CA Puente-Garza, S García-Lara - Planta, 2018 - Springer
Main conclusion Plant tissue culture as an important tool for the continuous production of
active compounds including secondary metabolites and engineered molecules. Novel …

Advancing crop transformation in the era of genome editing

F Altpeter, NM Springer, LE Bartley, AE Blechl… - The Plant …, 2016 - academic.oup.com
Plant transformation has enabled fundamental insights into plant biology and revolutionized
commercial agriculture. Unfortunately, for most crops, transformation and regeneration …

[HTML][HTML] Agrobacterium-mediated plant transformation: biology and applications

HH Hwang, M Yu, EM Lai - The arabidopsis book, 2017 - ncbi.nlm.nih.gov
Plant genetic transformation heavily relies on the bacterial pathogen Agrobacterium
tumefaciens as a powerful tool to deliver genes of interest into a host plant. Inside the plant …

[HTML][HTML] Methods and applications of CRISPR-mediated base editing in eukaryotic genomes

GT Hess, J Tycko, D Yao, MC Bassik - Molecular cell, 2017 - cell.com
The past several years have seen an explosion in development of applications for the
CRISPR-Cas9 system, from efficient genome editing, to high-throughput screening, to …

Floral dip: a simplified method for Agrobacterium ‐mediated transformation of Arabidopsis thaliana

SJ Clough, AF Bent - The plant journal, 1998 - Wiley Online Library
The Agrobacterium vacuum infiltration method has made it possible to transform Arabidopsis
thaliana without plant tissue culture or regeneration. In the present study, this method was …

Integration of Agrobacterium T-DNA into the Plant Genome

SB Gelvin - Annual review of genetics, 2017 - annualreviews.org
Agrobacterium strains transfer a single-strand form of T-DNA (T-strands) and Virulence (Vir)
effector proteins to plant cells. Following transfer, T-strands likely form complexes with Vir …

Agrobacterium-Mediated Plant Transformation: the Biology behind the “Gene-Jockeying” Tool

SB Gelvin - Microbiology and molecular biology reviews, 2003 - Am Soc Microbiol
Agrobacterium tumefaciens and related Agrobacterium species have been known as plant
pathogens since the beginning of the 20th century. However, only in the past two decades …

Optimization of Agrobacterium‐mediated transient assays of gene expression in lettuce, tomato and Arabidopsis

T Wroblewski, A Tomczak… - Plant Biotechnology …, 2005 - Wiley Online Library
Agrobacterium‐mediated transient assays for gene function are increasingly being used as
alternatives to genetic complementation and stable transformation. However, such assays …

Agrobacterium and plant genes involved in T-DNA transfer and integration

SB Gelvin - Annual review of plant biology, 2000 - annualreviews.org
▪ Abstract The phytopathogenic bacterium Agrobacterium tumefaciens genetically transforms
plants by transferring a portion of the resident Ti-plasmid, the T-DNA, to the plant …

Agrobacterium tumefaciens as an agent of disease

MA Escobar, AM Dandekar - Trends in plant science, 2003 - cell.com
Twenty-six years ago it was found that the common soil bacterium Agrobacterium
tumefaciens is capable of extraordinary feats of interkingdom genetic transfer. Since this …