Development of automated patch clamp assays to overcome the burden of variants of uncertain significance in inheritable arrhythmia syndromes

JG Ma, JI Vandenberg, CA Ng - Frontiers in Physiology, 2023 - frontiersin.org
Advances in next-generation sequencing have been exceptionally valuable for identifying
variants in medically actionable genes. However, for most missense variants there is …

Joint genotypic and phenotypic outcome modeling improves base editing variant effect quantification

J Ryu, S Barkal, T Yu, M Jankowiak, Y Zhou… - Nature Genetics, 2024 - nature.com
CRISPR base editing screens enable analysis of disease-associated variants at scale;
however, variable efficiency and precision confounds the assessment of variant-induced …

Activity-based selection for enhanced base editor mutational scanning

EG Kaplan, RJ Steger, ST Shah, LM Drepanos… - bioRxiv, 2024 - biorxiv.org
Base editing is a CRISPR-based technology that enables high-throughput, nucleotide-level
functional interrogation of the genome, which is essential for understanding the genetic …