Conformational dynamics of intrinsically disordered proteins regulate biomolecular condensate chemistry

A Abyzov, M Blackledge, M Zweckstetter - Chemical Reviews, 2022 - ACS Publications
Motions in biomolecules are critical for biochemical reactions. In cells, many biochemical
reactions are executed inside of biomolecular condensates formed by ultradynamic …

Hierarchical materials from high information content macromolecular building blocks: construction, dynamic interventions, and prediction

L Shao, J Ma, JL Prelesnik, Y Zhou, M Nguyen… - Chemical …, 2022 - ACS Publications
Hierarchical materials that exhibit order over multiple length scales are ubiquitous in nature.
Because hierarchy gives rise to unique properties and functions, many have sought …

Extreme dynamics in a biomolecular condensate

N Galvanetto, MT Ivanović, A Chowdhury, A Sottini… - Nature, 2023 - nature.com
Proteins and nucleic acids can phase-separate in the cell to form concentrated biomolecular
condensates,,–. The functions of condensates span many length scales: they modulate …

Deciphering how naturally occurring sequence features impact the phase behaviours of disordered prion-like domains

A Bremer, M Farag, WM Borcherds, I Peran… - Nature …, 2022 - nature.com
Prion-like low-complexity domains (PLCDs) have distinctive sequence grammars that
determine their driving forces for phase separation. Here we uncover the physicochemical …

Fixation can change the appearance of phase separation in living cells

S Irgen-Gioro, S Yoshida, V Walling, S Chong - elife, 2022 - elifesciences.org
Fixing cells with paraformaldehyde (PFA) is an essential step in numerous biological
techniques as it is thought to preserve a snapshot of biomolecular transactions in living cells …

Simulation of FUS protein condensates with an adapted coarse-grained model

Z Benayad, S von Bulow, LS Stelzl… - Journal of chemical …, 2020 - ACS Publications
Disordered proteins and nucleic acids can condense into droplets that resemble the
membraneless organelles observed in living cells. MD simulations offer a unique tool to …

Liquid–liquid phase separation modifies the dynamic properties of intrinsically disordered proteins

S Guseva, V Schnapka, W Adamski… - Journal of the …, 2023 - ACS Publications
Liquid–liquid phase separation of flexible biomolecules has been identified as a ubiquitous
phenomenon underlying the formation of membraneless organelles that harbor a multitude …

Molecular and environmental determinants of biomolecular condensate formation

JA Villegas, M Heidenreich, ED Levy - Nature chemical biology, 2022 - nature.com
Biomolecular condensate formation has been implicated in a host of biological processes
and has found relevance in biology and disease. Understanding the physical principles and …

Aging can transform single-component protein condensates into multiphase architectures

A Garaizar, JR Espinosa, JA Joseph… - Proceedings of the …, 2022 - National Acad Sciences
Phase-separated biomolecular condensates that contain multiple coexisting phases are
widespread in vitro and in cells. Multiphase condensates emerge readily within …

Expanding the molecular language of protein liquid–liquid phase separation

S Rekhi, CG Garcia, M Barai, A Rizuan, BS Schuster… - Nature Chemistry, 2024 - nature.com
Understanding the relationship between a polypeptide sequence and its phase separation
has important implications for analysing cellular function, treating disease and designing …