Light‐Responsive Materials in Droplet Manipulation for Biochemical Applications
Miniaturized droplets, characterized by well‐controlled microenvironments and capability for
parallel processing, have significantly advanced the studies on enzymatic evolution …
parallel processing, have significantly advanced the studies on enzymatic evolution …
Non-viral vectors for chimeric antigen receptor immunotherapy
US Tretbar, JG Rurik, EH Rustad, D Sürün… - Nature Reviews …, 2024 - nature.com
Chimeric antigen receptor (CAR) T cell therapy has demonstrated remarkable efficacy in
haematological malignancies. However, although viral vector-based methods are effective …
haematological malignancies. However, although viral vector-based methods are effective …
Genetically stable and scalable nanoengineering of human primary T cells via cell mechanoporation
Effective tumor regression has been observed with chimeric antigen receptor (CAR) T cells;
however, the development of an affordable, safe, and effective CAR-T cell treatment remains …
however, the development of an affordable, safe, and effective CAR-T cell treatment remains …
Multiplexed high-throughput localized electroporation workflow with deep learning–based analysis for cell engineering
Manipulation of cells for applications such as biomanufacturing and cell-based therapeutics
involves introducing biomolecular cargoes into cells. However, successful delivery is a …
involves introducing biomolecular cargoes into cells. However, successful delivery is a …
Electroactive nanoinjection platform for intracellular delivery and gene silencing
AR Shokouhi, Y Chen, HZ Yoh, T Murayama… - Journal of …, 2023 - Springer
Background Nanoinjection—the process of intracellular delivery using vertically configured
nanostructures—is a physical route that efficiently negotiates the plasma membrane, with …
nanostructures—is a physical route that efficiently negotiates the plasma membrane, with …
Advances in microfluidic strategies for single-cell research
Y Liu, Z Fan, L Qiao, B Liu - TrAC Trends in Analytical Chemistry, 2022 - Elsevier
A core challenge in biomedical and life science research is to decipher cell-to-cell
differences under pathological progression or external perturbation. Microfluidics-based …
differences under pathological progression or external perturbation. Microfluidics-based …
Sonoporation: Past, present, and future
A surge of research in intracellular delivery technologies is underway with the increased
innovations in cell‐based therapies and cell reprogramming. Particularly, physical cell …
innovations in cell‐based therapies and cell reprogramming. Particularly, physical cell …
Approaches and materials for endocytosis-independent intracellular delivery of proteins
N Chen, Y He, M Zang, Y Zhang, H Lu, Q Zhao… - Biomaterials, 2022 - Elsevier
The intracellular delivery of proteins is of great significance. For diseases such as cancer,
heart disease and neurodegenerative diseases, many important pharmacological targets …
heart disease and neurodegenerative diseases, many important pharmacological targets …
Electrotransfer for nucleic acid and protein delivery
Electrotransfer of nucleic acids and proteins has become crucial in biotechnology for gene
augmentation and genome editing. This review explores the applications of electrotransfer …
augmentation and genome editing. This review explores the applications of electrotransfer …
Delivering the CRISPR/Cas9 system for engineering gene therapies: Recent cargo and delivery approaches for clinical translation
RA Foley, RA Sims, EC Duggan, JK Olmedo… - … in Bioengineering and …, 2022 - frontiersin.org
Clustered Regularly Interspaced Short Palindromic Repeats associated protein 9
(CRISPR/Cas9) has transformed our ability to edit the human genome selectively. This …
(CRISPR/Cas9) has transformed our ability to edit the human genome selectively. This …