Agarose and its derivatives as supports for enzyme immobilization

P Zucca, R Fernandez-Lafuente, E Sanjust - Molecules, 2016 - mdpi.com
Agarose is a polysaccharide obtained from some seaweeds, with a quite particular structure
that allows spontaneous gelation. Agarose-based beads are highly porous, mechanically …

[HTML][HTML] Highly effective enzymes immobilization on ceramics: Requirements for supports and enzymes

J Kujawa, M Głodek, G Li, S Al-Gharabli… - Science of The Total …, 2021 - Elsevier
Enzyme immobilization is a well-known method for the improvement of enzyme reusability
and stability. To achieve very high effectiveness of the enzyme immobilization, not only does …

Trends in the development of innovative nanobiocatalysts and their application in biocatalytic transformations

E Gkantzou, AV Chatzikonstantinou, R Fotiadou… - Biotechnology …, 2021 - Elsevier
The ever-growing demand for cost-effective and innocuous biocatalytic transformations has
prompted the rational design and development of robust biocatalytic tools. Enzyme …

Strategies, challenges and opportunities of enzyme immobilization on porous silicon for biosensing applications

R Fopase, S Paramasivam, P Kale… - Journal of Environmental …, 2020 - Elsevier
The use of porous silicon (PS) for biosensing applications is now emerging as it offers
desired properties such as better surface-volume ratio, tunable pore size, ease of …

Enhanced enzyme stability through site-directed covalent immobilization

JCY Wu, CH Hutchings, MJ Lindsay, CJ Werner… - Journal of …, 2015 - Elsevier
Breakthroughs in enzyme immobilization have enabled increased enzyme recovery and
reusability, leading to significant decreases in the cost of enzyme use and fueling …

Immobilization of enzymes on functionalized cellulose nanofibrils for bioremediation of antibiotics: Degradation mechanism, kinetics, and thermodynamic study

MN Galodiya, S Chakma - Chemosphere, 2024 - Elsevier
The deteriorating environmental conditions due to increasing emerging recalcitrant
pollutants raised a severe concern for its remediation. In this study, we have reported …

Immobilization of thiol-modified horseradish peroxidase on gold nanoparticles enhances enzyme stability and prevents proteolytic digestion

FE Breausche, A Somerlot, J Walder, K Osei, S Okyem… - Langmuir, 2024 - ACS Publications
The specificity and efficiency of enzyme-mediated reactions have the potential to positively
impact many biotechnologies; however, many enzymes are easily degraded. Immobilization …

Use of chitosan heterofunctionality for enzyme immobilization: β-galactosidase immobilization for galacto-oligosaccharide synthesis

P Urrutia, C Bernal, L Wilson, A Illanes - International journal of biological …, 2018 - Elsevier
Chitosan partially functionalized with aldehyde groups was used for enzyme immobilization,
favoring first the enzyme adsorption through its amino groups and then the covalent bonding …

Protein crosslinking: uses in chemistry, biology and biotechnology

MN Gupta, M Perwez, M Sardar - Biocatalysis and …, 2020 - Taylor & Francis
Protein crosslinking is a part of many biological processes and is also carried out in vitro
under several controllable conditions with the help of any of the commercially available …

Enantiodivergent biosynthesis of β-hydroxy esters by self-sufficient heterogeneous biocatalysts in a continuous flow

D Andrés-Sanz, A Maiz-Iginitz, JM Bolivar… - Green …, 2024 - pubs.rsc.org
β-Hydroxy esters are essential building blocks utilised by the pharmaceutical and food
industries in the synthesis of functional products. The asymmetric reduction of β-keto esters …