Detergent-free isolation of membrane proteins and strategies to study them in a near-native membrane environment

B Krishnarjuna, A Ramamoorthy - Biomolecules, 2022 - mdpi.com
Atomic-resolution structural studies of membrane-associated proteins and peptides in a
membrane environment are important to fully understand their biological function and the …

Alzheimer's disease: The role of proteins in formation, mechanisms, and new therapeutic approaches

A Gholami - Neuroscience Letters, 2023 - Elsevier
Alzheimer's disease (AD) is a progressive neurological disorder that affects the central
nervous system (CNS), leading to memory and cognitive decline. In AD, the brain …

Enhancing the stability and homogeneity of non-ionic polymer nanodiscs by tuning electrostatic interactions

B Krishnarjuna, J Marte, T Ravula… - Journal of colloid and …, 2023 - Elsevier
The nanodisc technology is increasingly used for structural studies on membrane proteins
and drug delivery. The development of synthetic polymer nanodiscs and the recent …

Sulfonated polystyrenes: pH and Mg2+-insensitive amphiphilic copolymers for detergent-free membrane protein isolation

M Janata, S Gupta, E Čadová, P Angelisová… - European Polymer …, 2023 - Elsevier
Amphiphilic polymers are increasingly applied in the detergent-free isolation and functional
studies of membrane proteins. However, the carboxylate group present in the structure of …

Nanogels, nanodiscs, yeast cells, and metallo-complexes-based curcumin delivery for therapeutic applications

M Rahmanian, F Oroojalian, E Pishavar… - European Polymer …, 2023 - Elsevier
Curcumin extracted from the rhizomes of Curcuma longa and widely used because of its
biological advantages such as anti-inflammatory, anti-cancer, anti-oxidant, anti-ageing, anti …

[HTML][HTML] Characterization of size-tuneable aescin-lipid nanoparticles as platform for stabilization of membrane proteins

F Escobedo, M Gospalswamy, P Hägerbäumer… - Colloids and Surfaces B …, 2024 - Elsevier
Disc-like lipid nanoparticles stabilized by saponin biosurfactants display fascinating
properties, including their temperature-driven re-organization. β-Aescin, a saponin from …

An Acrylonitrile‐Based Copolymer Gel as an NMR Alignment Medium for Extraction of Residual Dipolar Couplings of Small Molecules in Aqueous Solution

DS Carvalho, DGB da Silva, F Hallwass… - …, 2023 - Wiley Online Library
An NMR weakly‐aligning polymer gel has been prepared by copolymerization of
acrylonitrile and 2‐acrylamide‐2‐methyl‐1‐propanesulfonic acid in the presence of 1, 4 …

Polymer-nanodiscs as a novel alignment medium for high-resolution NMR-based structural studies of nucleic acids

B Krishnarjuna, T Ravula, EM Faison, M Tonelli… - Biomolecules, 2022 - mdpi.com
Residual dipolar couplings (RDCs) are increasingly used for high-throughput NMR-based
structural studies and to provide long-range angular constraints to validate and refine …

Solid-state NMR methods for the characterization of bioconjugations and protein-material interactions

L Cerofolini, G Parigi, E Ravera, M Fragai… - Solid State Nuclear …, 2022 - Elsevier
Protein solid-state NMR has evolved dramatically over the last two decades, with the
development of new hardware and sample preparation methodologies. This technique is …

[HTML][HTML] Decomposition of mixed DMPC-aescin vesicles to bicelles is linked to the lipid's main phase transition: A direct evidence by using chain-deuterated lipid

C Dargel, LH Moleiro, A Radulescu, TJ Stank… - Journal of Colloid and …, 2025 - Elsevier
This work investigates the conversion of bicelles into larger sheets or closed vesicles upon
dilution and temperature increase for a system composed of the phospholipid 1, 2 …