Real-time PCR is the method of choice in many laboratories for diagnostic and food
applications. This technology merges the polymerase chain reaction chemistry with the use …

Abstract Background Quantitative PCR (qPCR) aims to measure the DNA or RNA
concentration in diagnostic and biological samples based on the quantification cycle (Cq) …

Anaerobic co-digestion (co-AD) could be a more sustainable waste management solution by
sharing the existed anaerobic digestion (AD) facilities and generating more biogas energy …

Background The analyses of amplification and melting curves have been shown to provide
valuable information on the quality of the individual reactions in quantitative PCR (qPCR) …

Real-time quantitative polymerase-chain-reaction (qPCR) is a standard technique in most
laboratories used for various applications in basic research. Analysis of qPCR data is a …

Sludge from wastewater treatment plants can act as a repository and crucial environmental
provider of antibiotic resistance genes (ARGs). Over the past few years, people's knowledge …

Abstract Background The Friedman rank sum test is a widely-used nonparametric method in
computational biology. In addition to examining the overall null hypothesis of no significant …

Mitochondrial dysfunction and inflammation contribute to the initiation and development of
several brain pathological conditions, including Alzheimer's disease and cerebral ischemia …

Quantitative PCR allows the precise measurement of DNA concentrations and is generally
considered to be straightforward and trouble free. However, a survey with 93 validated …

Circulating cell-free tumor DNA (ctDNA) is a promising biomarker in cancer. Ultrasensitive
technologies enable detection of low (< 0.1%) mutant allele frequencies, a pre-requisite to …