Role of Fc core fucosylation in the effector function of IgG1 antibodies

J Golay, AE Andrea, I Cattaneo - Frontiers in Immunology, 2022 - frontiersin.org
The presence of fucose on IgG1 Asn-297 N-linked glycan is the modification of the human
IgG1 Fc structure with the most significant impact on FcɣRIII affinity. It also significantly …

Glycosylation of immunoglobin G in tumors: Function, regulation and clinical implications

S Yang, M Cui, Q Liu, Q Liao - Cancer Letters, 2022 - Elsevier
Immunoglobulin G (IgG) is the predominant component in humoral immunity and the major
effector of neutralizing heterogeneous antigens. Glycosylation, as excessive …

Flux balance analysis and peptide map** elucidate the impact of bioreactor pH on Chinese hamster ovary (CHO) cell metabolism and N-linked glycosylation in the …

JV Reddy, SK Singh, T Leibiger, KH Lee… - Metabolic …, 2025 - Elsevier
Culture conditions have a profound impact on therapeutic protein production and
glycosylation, a critical therapeutic-quality attribute, especially for monoclonal antibodies …

Engineering nucleotide sugar synthesis pathways for independent and simultaneous modulation of N-glycan galactosylation and fucosylation in CHO cells

A Prabhu, D Shanmugam, M Gadgil - Metabolic Engineering, 2022 - Elsevier
Glycosylation of recombinant therapeutics like monoclonal antibodies (mAbs) is a critical
quality attribute. N-glycans in mAbs are known to affect various effector functions, and …

[PDF][PDF] An engineered immunomodulatory IgG1 Fc suppresses autoimmune inflammation through pathways shared with iv immunoglobulin

SL Sneed, BB Reese, AFS Laureano… - Journal of Clinical …, 2024 - Am Soc Clin Investig
Immunoglobulin G (IgG) antibodies in the form of high-dose intravenous immunoglobulin
(IVIG) exert immunomodulatory activity and are used in this capacity to treat inflammatory …

[HTML][HTML] Biomanufacturing of glycosylated antibodies: Challenges, solutions, and future prospects

KK Dubey, A Kumar, A Baldia, D Rajput… - Biotechnology …, 2023 - Elsevier
Traditionally, recombinant protein production has been done in several expression hosts of
bacteria, fungi, and majorly CHO (Chinese Hamster Ovary) cells; few have high production …

A Novel, Site‐Specific N‐Linked Glycosylation Model Provides Mechanistic Insights Into the Process‐Condition Dependent Distinct Fab and Fc Glycosylation of an …

JV Reddy, T Leibiger, SK Singh, KH Lee… - Biotechnology and …, 2024 - Wiley Online Library
The CHO VRC01 cell line produces an anti‐HIV IgG1 monoclonal antibody containing N‐
linked glycans on both the Fab (variable) and Fc (constant) regions. Site‐specific glycan …

Recent advances in antibody glycoengineering for the gain of functions

Z Liu, X Zou, F Tang, W Huang - Current Opinion in Chemical Biology, 2024 - Elsevier
Glycans play important roles in antibody functions, and antibody glycoengineering has long
been an important research field. Here, we summarize the significant strategies of antibody …

[HTML][HTML] Combination of sodium butyrate and decitabine promotes transgene expression in CHO cells via apoptosis inhibition

W Li, Z Fan, X Wang, Y Lin, TY Wang - New Biotechnology, 2022 - Elsevier
Chinese hamster ovary (CHO) cells are currently the most widely used host cells for
production of recombinant therapeutic proteins (RTPs). Small-molecule additives related to …

Comprehensive N-and O-glycoproteomic analysis of multiple Chinese hamster ovary host cell lines

Q Wang, T Wang, WW Wu, CY Lin, S Yang… - Journal of Proteome …, 2022 - ACS Publications
Glycoproteomic analysis of three Chinese hamster ovary (CHO) suspension host cell lines
(CHO-K1, CHO-S, and CHO-Pro5) commonly utilized in biopharmaceutical settings for …