Single-cell technologies, particularly single-cell RNA sequencing (scRNA-seq) methods,
together with associated computational tools and the growing availability of public data …

The recent boom in microfluidics and combinatorial indexing strategies, combined with low
sequencing costs, has empowered single-cell sequencing technology. Thousands—or even …

Rapid progress in the development of next-generation sequencing (NGS) technologies in
recent years has provided many valuable insights into complex biological systems, ranging …

The biggest hurdle to targeted cancer therapy is the inevitable emergence of drug
resistance. Tumor cells employ different mechanisms to resist the targeting agent. Most …

Characterizing the transcriptome of individual cells is fundamental to understanding
complex biological systems. We describe a droplet-based system that enables 3′ mRNA …

Tumor mutational burden and heterogeneity has been suggested to fuel resistance to many
targeted therapies. The cytosine deaminase APOBEC proteins have been implicated in the …

Single-cell transcriptome profiling of tumour tissue isolates allows the characterization of
heterogeneous tumour cells along with neighbouring stromal and immune cells. Here we …

Cultured cell lines are the workhorse of cancer research, but the extent to which they
recapitulate the heterogeneity observed among malignant cells in tumors is unclear. Here …

Pericytes are vascular mural cells embedded in the basement membrane of blood
microvessels. They extend their processes along capillaries, pre-capillary arterioles and …

Patient-derived xenografts (PDXs) have emerged as an important platform to elucidate new
treatments and biomarkers in oncology. PDX models are used to address clinically relevant …