Advances in CRISPR therapeutics

M Chavez, X Chen, PB Finn, LS Qi - Nature Reviews Nephrology, 2023 - nature.com
The clustered regularly interspaced short palindromic repeats (CRISPR) renaissance was
catalysed by the discovery that RNA-guided prokaryotic CRISPR-associated (Cas) proteins …

CRISPR technologies and the search for the PAM-free nuclease

D Collias, CL Beisel - Nature communications, 2021 - nature.com
The ever-expanding set of CRISPR technologies and their programmable RNA-guided
nucleases exhibit remarkable flexibility in DNA targeting. However, this flexibility comes with …

Plant breeding advancements with “CRISPR-Cas” genome editing technologies will assist future food security

M Ahmad - Frontiers in plant science, 2023 - frontiersin.org
Genome editing techniques are being used to modify plant breeding, which might increase
food production sustainably by 2050. A product made feasible by genome editing is …

Engineered probiotics

J Ma, Y Lyu, X Liu, X Jia, F Cui, X Wu, S Deng… - Microbial cell …, 2022 - Springer
Engineered probiotics are a kind of new microorganisms produced by modifying original
probiotics through gene editing. With the continuous development of tools and technology …

Rapid DNA unwinding accelerates genome editing by engineered CRISPR-Cas9

AR Eggers, K Chen, KM Soczek, OT Tuck, EE Doherty… - Cell, 2024 - cell.com
Thermostable clustered regularly interspaced short palindromic repeats (CRISPR) and
CRISPR-associated (Cas9) enzymes could improve genome-editing efficiency and delivery …

Sensitive and portable signal readout strategies boost point-of-care CRISPR/Cas12a biosensors

T Li, N Cheng - ACS sensors, 2023 - ACS Publications
Point-of-care (POC) detection is getting more and more attention in many fields due to its
accuracy and on-site test property. The CRISPR/Cas12a system is endowed with excellent …

A review on advanced CRISPR-based genome-editing tools: base editing and prime editing

A Saber Sichani, M Ranjbar, M Baneshi… - Molecular …, 2023 - Springer
In the field of medicine, it is axiomatic that the need of a precise gene-editing tool is critical to
employ therapeutic approaches toward pathogenic mutations, occurring in human genome …

Nucleic acid-assisted CRISPR-Cas systems for advanced biosensing and bioimaging

S Chen, B Gong, C Zhu, C Lei, Z Nie - TrAC Trends in Analytical Chemistry, 2023 - Elsevier
Nucleic acid molecules possess many superior properties, including high designability and
structural predictability, making them attractive biosensing and bioimaging tools. The …

Genome editing and beyond: what does it mean for the future of plant breeding?

T Van Vu, S Das, G Hensel, JY Kim - Planta, 2022 - Springer
Main conclusion Genome editing offers revolutionized solutions for plant breeding to sustain
food production to feed the world by 2050. Therefore, genome-edited products are …

CRISPR/Cas9 in lepidopteran insects: Progress, application and prospects

JJ Li, Y Shi, JN Wu, H Li, G Smagghe, TX Liu - Journal of insect physiology, 2021 - Elsevier
Clustered regularly spaced short palindrome repeats (CRISPR) structure family forms the
acquired immune system in bacteria and archaea. Recent advances in CRISPR/Cas …