Developments in hydrogen/deuterium exchange mass spectrometry

JR Engen, T Botzanowski, D Peterle… - Analytical …, 2020 - ACS Publications
Chemistry” on HDX MS. 1 That review covered HDX MS applications from January 2012
through June 2014 and described the publications in terms of sector, geography, and topic …

Small-angle X-ray scattering studies of enzymes

AS Byer, X Pei, MG Patterson, N Ando - Current opinion in chemical biology, 2023 - Elsevier
Enzyme function requires conformational changes to achieve substrate binding, domain
rearrangements, and interactions with partner proteins, but these movements are difficult to …

A High‐Affinity Calmodulin‐Binding Site in the CyaA Toxin Translocation Domain is Essential for Invasion of Eukaryotic Cells

A Voegele, M Sadi, DP O'Brien, P Gehan… - Advanced …, 2021 - Wiley Online Library
The molecular mechanisms and forces involved in the translocation of bacterial toxins into
host cells are still a matter of intense research. The adenylate cyclase (CyaA) toxin from …

Perspectives on solution-based small angle X-ray scattering for protein and biological macromolecule structural biology

ASA Mohammed, D Soloviov… - Physical Chemistry …, 2024 - pubs.rsc.org
Small-angle X-rays scattering (SAXS) is used to extract structural information from a wide
variety of non-crystalline samples in different fields (eg, materials science, physics …

Calmodulin fishing with a structurally disordered bait triggers CyaA catalysis

DP O'Brien, D Durand, A Voegele, V Hourdel… - PLoS …, 2017 - journals.plos.org
Once translocated into the cytosol of target cells, the catalytic domain (AC) of the adenylate
cyclase toxin (CyaA), a major virulence factor of Bordetella pertussis, is potently activated by …

Biophysical characterisation of human LincRNA-p21 sense and antisense Alu inverted repeats

MH D'Souza, T Mrozowich, MD Badmalia… - Nucleic acids …, 2022 - academic.oup.com
Abstract Human Long Intergenic Noncoding RNA-p21 (LincRNA-p21) is a regulatory
noncoding RNA that plays an important role in promoting apoptosis. LincRNA-p21 is also …

Post‐translational acylation controls the folding and functions of the CyaA RTX toxin

DP O'Brien, SE Cannella, A Voegele… - The FASEB …, 2019 - Wiley Online Library
The adenylate cyclase (CyaA) toxin is a major virulence factor of Bordetella pertussis, the
causative agent of whoo** cough. CyaA is synthetized as a pro‐toxin, pro‐CyaA, and …

Interpreting hydrogen-deuterium exchange experiments with molecular simulations: Tutorials and applications of the HDXer ensemble reweighting software [Article v1 …

PS Lee, RT Bradshaw, F Marinelli… - Living journal of …, 2022 - pmc.ncbi.nlm.nih.gov
Hydrogen-deuterium exchange (HDX) is a comprehensive yet detailed probe of protein
structure and dynamics and, coupled to mass spectrometry, has become a powerful tool for …

Bioengineering of Bordetella pertussis Adenylate Cyclase Toxin for Antigen-Delivery and Immunotherapy

A Chenal, D Ladant - Toxins, 2018 - mdpi.com
The adenylate cyclase toxin (CyaA) is one of the major virulence factors of Bordetella
pertussis, the causative agent of whoo** cough. CyaA is able to invade eukaryotic cells …
[Free GPT-4]
K **ao, Y Zhao, M Choi, H Liu, A Blanc, J Qian… - nature protocols, 2018 - nature.com
Many cellular functions necessitate structural assemblies of two or more associated proteins.
The structural characterization of protein complexes using standard methods, such as X-ray …