Multiplexed electrochemical assays for clinical applications

M Sharafeldin, JF Rusling - Current opinion in electrochemistry, 2023 - Elsevier
Rapid, accurate diagnoses are central to future efficient healthcare to identify diseases at
early stages, avoid unnecessary treatment, and improve outcomes. Electrochemical …

Synthetic antigen-conjugated dna systems for antibody detection and characterization

S Ranallo, S Bracaglia, D Sorrentino, F Ricci - ACS sensors, 2023 - ACS Publications
Antibodies are among the most relevant biomolecular targets for diagnostic and clinical
applications. In this Perspective, we provide a critical overview of recent research efforts …

Electrochemical cell‐free biosensors for antibody detection

S Bracaglia, S Ranallo, F Ricci - Angewandte Chemie, 2023 - Wiley Online Library
We report here the development of an electrochemical cell‐free biosensor for antibody
detection directly in complex sample matrices with high sensitivity and specificity that is …

PAM‐Engineered Toehold Switches as Input‐Responsive Activators of CRISPR‐Cas12a for Sensing Applications

N Bagheri, A Chamorro, A Idili… - Angewandte Chemie, 2024 - Wiley Online Library
The RNA‐programmed CRISPR effector protein Cas12a has emerged as a powerful tool for
gene editing and molecular diagnostics. However, additional bio‐engineering strategies are …

Programmable cell-free transcriptional switches for antibody detection

A Patino Diaz, S Bracaglia, S Ranallo… - Journal of the …, 2022 - ACS Publications
We report here the development of a cell-free in vitro transcription system for the detection of
specific target antibodies. The approach is based on the use of programmable antigen …

Amplification-free ratiometric electrochemical aptasensor for point-of-care detection of therapeutic monoclonal antibodies

J Wan, Y Liang, Q Hu, Z Liang, W Feng, Y Tian… - Analytical …, 2023 - ACS Publications
The rapid quantification of therapeutic monoclonal antibodies (mAbs) is of great significance
to their pharmacokinetics/pharmacodynamics (PK/PD) research and the personalized …

Enzyme‐Linked DNA Displacement (ELIDIS) Assay for Ultrasensitive Electrochemical Detection of Antibodies

A Díaz‐Fernández, S Ranallo… - Angewandte Chemie …, 2024 - Wiley Online Library
Here we report the development of a method for the electrochemical ultrasensitive detection
of antibodies that couples the programmability and versatility of DNA‐based systems with …

Single‐response duplexing of electrochemical label‐free biosensor from the same tag

JNY Costa, GJC Pimentel, JA Poker… - Advanced …, 2024 - Wiley Online Library
Multiplexing is a valuable strategy to boost throughput and improve clinical accuracy.
Exploiting the vertical, meshed design of reproducible and low‐cost ultra‐dense …

Programing chemical communication: allostery vs multivalent mechanism

D Lauzon, A Vallée-Bélisle - Journal of the American Chemical …, 2023 - ACS Publications
The emergence of life has relied on chemical communication and the ability to integrate
multiple chemical inputs into a specific output. Two mechanisms are typically employed by …

Wash-free, sandwich-type protein detection using direct electron transfer and catalytic signal amplification of multiple redox labels

G Kim, H Cho, P Nandhakumar, JK Park… - Analytical …, 2022 - ACS Publications
Direct electron transfer (DET) between a redox label and an electrode has been used for
sensitive and selective sandwich-type detection without a wash step. However, applying …