The native chemical ligation reaction (NCL) involves reacting a C-terminal peptide thioester
with an N-terminal cysteinyl peptide to produce a native peptide bond between the two …

Bioconjugation methodologies have proven to play a central enabling role in the recent
development of biotherapeutics and chemical biology approaches. Recent endeavours in …

This protocol provides a detailed procedure for the chemical synthesis of proteins through
native chemical ligation of peptide hydrazides. The two crucial stages of this protocol are (i) …

pH determines selectivity: The ligation of peptide hydrazides is a new method for protein
chemical synthesis that is complementary to native chemical ligation. Peptide hydrazides …

Coming together: A generally applicable strategy for convergent chemical synthesis of
proteins from multiple peptide segments is developed on the basis of the ligation of peptide …

Total chemical synthesis of proteins is usually achieved by assembling unprotected peptide
segments using site-specific and chemoselective native peptide ligation methods. Access to …

The chemical synthesis of proteins provides synthetic chemists with an interesting challenge
and supports biological research through the generation of proteins that are not produced …

Peptide thioesters play a key role in convergent protein synthesis strategies such as native
chemical ligation, traceless Staudinger ligation, and Ag+‐mediated thioester ligation. The …

We report here an efficient and mild method for constructing C–S bonds. The reactions were
carried out with Na2S2O3 as a sulfurating reagent, CuSO4 as a catalyst, and water as …

Protein chemical synthesis by native peptide ligation of unprotected peptide segments is an
interesting complement and potential alternative to the use of living systems for producing …