Recombinase polymerase amplification: Basics, applications and recent advances
IM Lobato, CK O'Sullivan - Trac Trends in analytical chemistry, 2018 - Elsevier
Recombinase polymerase amplification (RPA) is a highly sensitive and selective isothermal
amplification technique, operating at 37–42° C, with minimal sample preparation and …
amplification technique, operating at 37–42° C, with minimal sample preparation and …
A comprehensive summary of a decade development of the recombinase polymerase amplification
Nucleic acid amplification has permeated every field in the life sciences since the
introduction of the classic polymerase chain reaction (PCR) method in 1983. Yet, despite its …
introduction of the classic polymerase chain reaction (PCR) method in 1983. Yet, despite its …
Recombinase polymerase amplification for diagnostic applications
RK Daher, G Stewart, M Boissinot… - Clinical …, 2016 - academic.oup.com
BACKGROUND First introduced in 2006, recombinase polymerase amplification (RPA) has
stirred great interest, as evidenced by 75 publications as of October 2015, with 56 of them …
stirred great interest, as evidenced by 75 publications as of October 2015, with 56 of them …
Isothermal amplification of nucleic acids: The race for the next “gold standard”
Nucleic acid amplification technologies (NAATs) have become fundamental tools in
molecular diagnostics, due to their ability to detect small amounts of target molecules. Since …
molecular diagnostics, due to their ability to detect small amounts of target molecules. Since …
Onsite detection of plant viruses using isothermal amplification assays
Plant diseases caused by viruses limit crop production and quality, resulting in significant
losses. However, options for managing viruses are limited; for example, as systemic obligate …
losses. However, options for managing viruses are limited; for example, as systemic obligate …
CRISPR-based rapid and ultra-sensitive diagnostic test for Mycobacterium tuberculosis
JW Ai, X Zhou, T Xu, M Yang, Y Chen… - Emerging microbes & …, 2019 - Taylor & Francis
Rapid and simple-to-use diagnostic methods for tuberculosis are urgently needed. Recent
development has unveiled the diagnostic power of the CRISPR system in the detection of …
development has unveiled the diagnostic power of the CRISPR system in the detection of …
Advanced DNA-based point-of-care diagnostic methods for plant diseases detection
Diagnostic technologies for the detection of plant pathogens with point-of-care capability
and high multiplexing ability are an essential tool in the fight to reduce the large agricultural …
and high multiplexing ability are an essential tool in the fight to reduce the large agricultural …
Critical insight into recombinase polymerase amplification technology
MA Munawar - Expert Review of Molecular Diagnostics, 2022 - Taylor & Francis
Introduction Recombinase polymerase amplification (RPA) is a promising and emerging
technology for rapidly amplifying target nucleic acid from minimally processed samples and …
technology for rapidly amplifying target nucleic acid from minimally processed samples and …
Development and applications of portable biosensors
B Srinivasan, S Tung - Journal of laboratory automation, 2015 - journals.sagepub.com
The significance of microfluidics-based and microelectromechanical systems–based
biosensors has been widely acknowledged, and many reviews have explored their potential …
biosensors has been widely acknowledged, and many reviews have explored their potential …
Influence of sequence mismatches on the specificity of recombinase polymerase amplification technology
Recombinase polymerase amplification (RPA) technology relies on three major proteins,
recombinase proteins, single-strand binding proteins, and polymerases, to specifically …
recombinase proteins, single-strand binding proteins, and polymerases, to specifically …