Genome editing with CRISPR–Cas nucleases, base editors, transposases and prime editors
The development of new CRISPR–Cas genome editing tools continues to drive major
advances in the life sciences. Four classes of CRISPR–Cas-derived genome editing agents …
advances in the life sciences. Four classes of CRISPR–Cas-derived genome editing agents …
CRISPR in cancer biology and therapy
Over the past decade, CRISPR has become as much a verb as it is an acronym,
transforming biomedical research and providing entirely new approaches for dissecting all …
transforming biomedical research and providing entirely new approaches for dissecting all …
[HTML][HTML] A next generation connectivity map: L1000 platform and the first 1,000,000 profiles
A Subramanian, R Narayan, SM Corsello, DD Peck… - Cell, 2017 - cell.com
We previously piloted the concept of a Connectivity Map (CMap), whereby genes, drugs,
and disease states are connected by virtue of common gene-expression signatures. Here …
and disease states are connected by virtue of common gene-expression signatures. Here …
Autism genes converge on asynchronous development of shared neuron classes
B Paulsen, S Velasco, AJ Kedaigle, M Pigoni… - Nature, 2022 - nature.com
Genetic risk for autism spectrum disorder (ASD) is associated with hundreds of genes
spanning a wide range of biological functions,,,,–. The alterations in the human brain …
spanning a wide range of biological functions,,,,–. The alterations in the human brain …
Evolved Cas9 variants with broad PAM compatibility and high DNA specificity
A key limitation of the use of the CRISPR–Cas9 system for genome editing and other
applications is the requirement that a protospacer adjacent motif (PAM) be present at the …
applications is the requirement that a protospacer adjacent motif (PAM) be present at the …
High-fidelity CRISPR–Cas9 nucleases with no detectable genome-wide off-target effects
CRISPR–Cas9 nucleases are widely used for genome editing but can induce unwanted off-
target mutations. Existing strategies for reducing genome-wide off-target effects of the widely …
target mutations. Existing strategies for reducing genome-wide off-target effects of the widely …
Genome-wide CRISPR screens reveal host factors critical for SARS-CoV-2 infection
Identification of host genes essential for severe acute respiratory syndrome coronavirus 2
(SARS-CoV-2) infection may reveal novel therapeutic targets and inform our understanding …
(SARS-CoV-2) infection may reveal novel therapeutic targets and inform our understanding …
Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9
CRISPR-Cas9–based genetic screens are a powerful new tool in biology. By simply altering
the sequence of the single-guide RNA (sgRNA), one can reprogram Cas9 to target different …
the sequence of the single-guide RNA (sgRNA), one can reprogram Cas9 to target different …
Evaluation of off-target and on-target scoring algorithms and integration into the guide RNA selection tool CRISPOR
M Haeussler, K Schönig, H Eckert, A Eschstruth… - Genome biology, 2016 - Springer
Background The success of the CRISPR/Cas9 genome editing technique depends on the
choice of the guide RNA sequence, which is facilitated by various websites. Despite the …
choice of the guide RNA sequence, which is facilitated by various websites. Despite the …
Perturb-Seq: dissecting molecular circuits with scalable single-cell RNA profiling of pooled genetic screens
Genetic screens help infer gene function in mammalian cells, but it has remained difficult to
assay complex phenotypes—such as transcriptional profiles—at scale. Here, we develop …
assay complex phenotypes—such as transcriptional profiles—at scale. Here, we develop …