The promise and challenge of high-throughput sequencing of the antibody repertoire
Efforts to determine the antibody repertoire encoded by B cells in the blood or lymphoid
organs using high-throughput DNA sequencing technologies have been advancing at an …
organs using high-throughput DNA sequencing technologies have been advancing at an …
Development of broadly neutralizing antibodies in HIV-1 infected elite neutralizers
Broadly neutralizing antibodies (bNAbs), able to prevent viral entry by diverse global
viruses, are a major focus of HIV vaccine design, with data from animal studies confirming …
viruses, are a major focus of HIV vaccine design, with data from animal studies confirming …
Tailored immunogens direct affinity maturation toward HIV neutralizing antibodies
Induction of broadly neutralizing antibodies (bnAbs) is a primary goal of HIV vaccine
development. VRC01-class bnAbs are important vaccine leads because their precursor B …
development. VRC01-class bnAbs are important vaccine leads because their precursor B …
The effects of somatic hypermutation on neutralization and binding in the PGT121 family of broadly neutralizing HIV antibodies
Broadly neutralizing HIV antibodies (bnAbs) are typically highly somatically mutated, raising
doubts as to whether they can be elicited by vaccination. We used 454 sequencing and …
doubts as to whether they can be elicited by vaccination. We used 454 sequencing and …
Immunosequencing: applications of immune repertoire deep sequencing
H Robins - Current opinion in immunology, 2013 - Elsevier
Highlights•New technology to profile the adaptive immune system with high-throughput
sequencing of adaptive immune receptors.•Ability to follow the dynamics of an adaptive …
sequencing of adaptive immune receptors.•Ability to follow the dynamics of an adaptive …
Analyzing immunoglobulin repertoires
Somatic assembly of T cell receptor and B cell receptor (BCR) genes produces a vast
diversity of lymphocyte antigen recognition capacity. The advent of efficient high-throughput …
diversity of lymphocyte antigen recognition capacity. The advent of efficient high-throughput …
Sequence-intrinsic mechanisms that target AID mutational outcomes on antibody genes
In activated B lymphocytes, AID initiates antibody variable (V) exon somatic hypermutation
(SHM) for affinity maturation in germinal centers (GCs) and IgH switch (S) region DNA …
(SHM) for affinity maturation in germinal centers (GCs) and IgH switch (S) region DNA …
Immunoglobulin gene insertions and deletions in the affinity maturation of HIV-1 broadly reactive neutralizing antibodies
TB Kepler, HX Liao, SM Alam, R Bhaskarabhatla… - Cell host & …, 2014 - cell.com
Induction of HIV-1 broad neutralizing antibodies (bnAbs) is a goal of HIV-1 vaccine
development but has remained challenging partially due to unusual traits of bnAbs …
development but has remained challenging partially due to unusual traits of bnAbs …
Related mechanisms of antibody somatic hypermutation and class switch recombination
JK Hwang, FW Alt, LS Yeap - Mobile DNA III, 2015 - Wiley Online Library
The B cell receptor (BCR) is expressed on the B lymphocyte cell surface where it serves as a
receptor for foreign antigens (1). The BCR is comprised of two immunoglobulin (Ig) heavy …
receptor for foreign antigens (1). The BCR is comprised of two immunoglobulin (Ig) heavy …
Insights into the structural basis of antibody affinity maturation from next-generation sequencing
Affinity maturation is the process whereby the immune system generates antibodies of
higher affinities during a response to antigen. It is unique in being the only evolutionary …
higher affinities during a response to antigen. It is unique in being the only evolutionary …