Gene drives are selfish genetic elements that are transmitted to progeny at super-Mendelian
(> 50%) frequencies. Recently developed CRISPR–Cas9-based gene-drive systems are …

Mosquito-borne diseases, such as dengue and malaria, pose significant global health
burdens. Unfortunately, current control methods based on insecticides and environmental …

In the human malaria vector Anopheles gambiae, the gene doublesex (Agdsx) encodes two
alternatively spliced transcripts, dsx-female (AgdsxF) and dsx-male (AgdsxM), that control …

Clustered, regularly interspaced short palindromic repeats (CRISPR) and CRISPR-
associated (Cas) genes, a diverse family of prokaryotic adaptive immune systems, have …

Only female insects transmit diseases such as malaria, dengue and Zika; therefore, control
methods that bias the sex ratio of insect offspring have long been sought. Genetic elements …

Targeted nucleases have provided researchers with the ability to manipulate virtually any
genomic sequence, enabling the facile creation of isogenic cell lines and animal models for …

Aedes mosquito-transmitted diseases, such as dengue, Zika and chikungunya, are
becoming major global health emergencies while old threats, such as yellow fever, are re …

Genetic engineering technologies can be used both to create transgenic mosquitoes
carrying antipathogen effector genes targeting human malaria parasites and to generate …

Engineered gene drives—the process of stimulating the biased inheritance of specific genes—
have the potential to enable the spread of desirable genes throughout wild populations or to …

Gene drive systems that enable super-Mendelian inheritance of a transgene have the
potential to modify insect populations over a timeframe of a few years. We describe CRISPR …