Bacterial cyclophane-containing RiPPs from radical SAM enzymes

CS Phan, BI Morinaka - Natural Product Reports, 2024 - pubs.rsc.org
Covering: 2016 to 2023 Ribosomally synthesized and posttranslationally modified peptides
(RiPPs) continue to be a rich source of chemically diverse and bioactive peptide natural …

Bacterial Cytochrome P450 Catalyzed Post‐translational Macrocyclization of Ribosomal Peptides

BB He, J Liu, Z Cheng, R Liu, Z Zhong… - Angewandte Chemie …, 2023 - Wiley Online Library
Ribosomally synthesized and post‐translationally modified peptides (RiPPs) are a
fascinating group of natural products that exhibit diverse structural features and bioactivities …

Substrate-controlled catalysis in the ether cross-link-forming radical SAM enzymes

S Ma, W **, S Wang, H Chen, S Guo, T Mo… - Journal of the …, 2023 - ACS Publications
Darobactin is a heptapeptide antibiotic featuring an ether cross-link and a C–C cross-link,
and both cross-links are installed by a radical S-adenosylmethionine (rSAM) enzyme DarE …

Discovery and engineering of ribosomally synthesized and post-translationally modified peptide (RiPP) natural products

H Li, W Ding, Q Zhang - RSC Chemical Biology, 2024 - pubs.rsc.org
Ribosomally synthesized and post-translationally modified peptides (RiPPs) represent a
diverse superfamily of natural products with immense potential for drug development. This …

Biosynthesis of macrocyclic peptides with C-terminal β-amino-α-keto acid groups by three different metalloenzymes

DT Nguyen, L Zhu, DL Gray, TJ Woods… - ACS Central …, 2024 - ACS Publications
Advances in genome sequencing and bioinformatics methods have identified a myriad of
biosynthetic gene clusters (BGCs) encoding uncharacterized molecules. By mining …

Catalytic Site Proximity Profiling for Functional Unification of Sequence-Diverse Radical S-Adenosylmethionine Enzymes

TW Precord, S Ramesh, SR Dommaraju… - ACS bio & med …, 2023 - ACS Publications
The radical S-adenosylmethionine (rSAM) superfamily has become a wellspring for
discovering new enzyme chemistry, especially regarding ribosomally synthesized and post …

Bacterial Cytochrome P450 Catalyzed Macrocyclization of Ribosomal Peptides

J Liu, R Liu, BB He, X Lin, L Guo, G Wu… - ACS Bio & Med Chem …, 2024 - ACS Publications
Macrocyclization is a vital process in the biosynthesis of ribosomally synthesized and post-
translationally modified peptides (RiPPs), significantly enhancing their structural diversity …

Rule-based omics mining reveals antimicrobial macrocyclic peptides against drug-resistant clinical isolates

Z Cheng, BB He, K Lei, Y Gao, Y Shi, Z Zhong… - Nature …, 2024 - nature.com
Antimicrobial resistance remains a significant global threat, driving up mortality rates
worldwide. Ribosomally synthesized and post-translationally modified peptides have …

Genome Mining for New Enzyme Chemistry

DT Nguyen, DA Mitchell, WA van der Donk - ACS catalysis, 2024 - ACS Publications
A revolution in the field of biocatalysis has enabled scalable access to compounds of high
societal values using enzymes. The construction of biocatalytic routes relies on the reservoir …

Aromatic side-chain crosslinking in RiPP biosynthesis

SK Kandy, MA Pasquale, JR Chekan - Nature Chemical Biology, 2025 - nature.com
Peptide cyclization is a defining feature of many bioactive molecules, particularly in the
ribosomally synthesized and post-translationally modified peptide (RiPP) family of natural …